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k/vol1/fastq/SRR236/050/SRR23674950/SRR23674950_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR236/062/SRR23674962/SRR23674962_1.fastq.gzprimaryOK200GenomicsAMRF Functional Genomics Common Research Resource, Department of Psychiatry and Neurology Department of Psychiatry, University of California, Los Angeleshttps://www.ebi.ac.uk/ena/browser/view/PRJNA940089Mus musculusxref:PubMed:37973910We generated adeno-associated Cre viral vectors with improved astrocyte-specific expression by including a cassette of miRNA targeting sequences (4x6T) to decrease off-target expression. We delivered these viruses in Ribotag mice using the PHP.eB serotype, either intracortically or systemically. We compared these samples with mice that received intracortical AAV2/5::GfaABC1D-Cre and transgenic Aldh1l1-CreERT2/Ribotag mice. We sequenced whole-tissue RNA as well as ribosomally-loaded astrocyte-enriched mRNA to assess cell-type specificity of viruses as well as virus-related transcriptomic changes. Overall design: Four cohorts were generated: Aldh1l1-CreERT2/Ribotag transgenic mice (control mice with highly astrocyte-specific labeled ribosomes) AAV2/5::GfaABC1D-Cre intracortical injection mice (standard astrocyte-enriched virus) PHP.eB::GfaABC1D-Cre-4x6T intracortical injection mice and PHP.eB::GfaABC1D-Cre-4x6T systemic injection mice. Each cohort included 4 mice, and each mouse had both input (whole-cortex) and immunoprecipitated (ribosomally loaded, astrocyte-enriched) mRNA samples.ENAfalseTranscriptional analysis of murine cortical astrocytes with adeno-associated viral transduction with and without 4x6T miR cassette, vs transgenically labeled astrocytesTranscriptional analysis of murine cortical astrocytes with adeno-associated viral transduction with and without 4x6T miR cassette, vs transgenically labeled astrocytes2025-09-242023-03-06PRJNA940089GSE2263661009037973910