ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/043/SRR23950943/SRR23950943_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/038/SRR23950938/SRR23950938_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/047/SRR23950947/SRR23950947_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/046/SRR23950946/SRR23950946_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/042/SRR23950942/SRR23950942_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/039/SRR23950939/SRR23950939_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/050/SRR23950950/SRR23950950_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/051/SRR23950951/SRR23950951_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/044/SRR23950944/SRR23950944_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/045/SRR23950945/SRR23950945_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/042/SRR23950942/SRR23950942_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/041/SRR23950941/SRR23950941_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/047/SRR23950947/SRR23950947_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/048/SRR23950948/SRR23950948_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/052/SRR23950952/SRR23950952_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/048/SRR23950948/SRR23950948_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/041/SRR23950941/SRR23950941_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/040/SRR23950940/SRR23950940_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/049/SRR23950949/SRR23950949_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/046/SRR23950946/SRR23950946_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/037/SRR23950937/SRR23950937_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/039/SRR23950939/SRR23950939_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/049/SRR23950949/SRR23950949_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/040/SRR23950940/SRR23950940_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/045/SRR23950945/SRR23950945_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/050/SRR23950950/SRR23950950_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/037/SRR23950937/SRR23950937_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/044/SRR23950944/SRR23950944_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/043/SRR23950943/SRR23950943_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/038/SRR23950938/SRR23950938_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/052/SRR23950952/SRR23950952_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR239/051/SRR23950951/SRR23950951_1.fastq.gzprimaryOK200GenomicsGenomic Technologies Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseaseshttps://www.ebi.ac.uk/ena/browser/view/PRJNA948056Mus musculusControl of Mycobacterium tuberculosis infection requires generation of T cells that migrate to granulomas, complex immune structures surrounding sites of bacterial replication. Here we compared the gene expression profiles of T cells in pulmonary granulomas, bronchoalveolar lavage and blood of Mtb-infected rhesus macaques to identify granuloma-enriched T cell genes. TNFRSF8/CD30 was among the top genes that was upregulated in both CD4 and CD8 granuloma T cells and independent of bacterial loads. Transcriptomic profiling of lung T cells from Mtb-infected mixed bone marrow chimeric mice showed that CD30 directly promotes CD4 T cell differentiation and effector molecule expression. Moreover, in mice CD30 expression on CD4 T cells is required for survival of Mtb infection. These results show the CD30 co-stimulatory axis is highly upregulated on granuloma T cells and is critical for the generation of protective T cell responses against Mtb infection. Overall design: RNA-seq of mouse CD4 T-cells. Balanced 2-factor design (WT/KO MTb Activated/ Naïve), N=4 mice per groupxref:PubMed:37097292ENAfalseGene expression profiling of granuloma T cells in M. tuberculosis infected rhesus macaques reveals a critical role for CD30 [Mus musculus]Gene expression profiling of granuloma T cells in M. tuberculosis infected rhesus macaques reveals a critical role for CD30 [Mus musculus]2025-09-242023-03-31PRJNA948056GSE2281131009037097292