{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/059/SRR24102359/SRR24102359_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/077/SRR28573177/SRR28573177_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/060/SRR24102360/SRR24102360_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/058/SRR24102358/SRR24102358_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/063/SRR24102363/SRR24102363_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/063/SRR24102363/SRR24102363_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/058/SRR24102358/SRR24102358_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/062/SRR24102362/SRR24102362_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/075/SRR28573175/SRR28573175_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/061/SRR24102361/SRR24102361_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/060/SRR24102360/SRR24102360_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/077/SRR28573177/SRR28573177_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/076/SRR28573176/SRR28573176_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/059/SRR24102359/SRR24102359_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/075/SRR28573175/SRR28573175_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/061/SRR24102361/SRR24102361_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/062/SRR24102362/SRR24102362_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/076/SRR28573176/SRR28573176_2.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Biomedical chemistry, School of Molecular and Cellular Biology, Graduate School of Medicine, The University of Tokyo"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA953347"],"scientific_name":["Entamoeba histolytica"],"long_description":["To know the role of Rho small GTPases of enteric protozoan parasite Entamoeba histolytica, ten Rho small GTPases were specifically silenced and examined the effect on virulence-related phenotypes. Among them, EhRacM gene silence caused an enhancement in macropinocytosis. To clarify transcriptomic difference in EhRacM gene silenceing strain, RNA-seq analysis was conducted. Overall design: To know the role of Rho small GTPases of enteric protozoan parasite Entamoeba histolytica, ten Rho small GTPases were specifically silenced and examined the effect on virulence-related phenotypes. Among them, EhracM gene silencing caused an enhancement in macropinocytosis. Also, EhracJ gene silencing caused a slight decrease in trogocytosis. To clarify the transcriptomic differences in EhracM gene silenced strain and EhracJ gene silenced strain, RNA-seq analysis was conducted."],"tag":["xref:PubMed:39536056","pathogen:protozoan","pathogen"],"classification":["protozoa"],"repository":["ENA"],"description_synonyms":["strain, epigenetic, gene silencing, Inactivation, Gene Inactivation, Strain, Sprain, Whole Transcriptome Shotgun Sequencing, Strain., RNA-seq, inhibition of gene expression, Gene, cultivar, Strains, Sprains, Strains and Sprains, Silencing, ecotype"],"name_synonyms":["strain, epigenetic, gene silencing, Inactivation, Gene Inactivation, Strain, Sprain, Whole Transcriptome Shotgun Sequencing, Strain., RNA-seq, inhibition of gene expression, Gene, cultivar, Strains, Sprains, Strains and Sprains, Silencing, ecotype"],"additional_accession":[]},"is_claimable":false,"name":"The results of RNA-seq for EhracM gene silencing strain and EhracJ gene silencing strain","description":"The results of RNA-seq for EhracM gene silencing strain and EhracJ gene silencing strain","dates":{"last_updated":"2025-09-24","first_public":"2024-06-22"},"accession":"PRJNA953347","cross_references":{"GEO":["GSE229171"],"taxon":["5759"],"PubMed":["39536056"]}}