ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/059/SRR24102359/SRR24102359_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/077/SRR28573177/SRR28573177_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/060/SRR24102360/SRR24102360_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/058/SRR24102358/SRR24102358_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/063/SRR24102363/SRR24102363_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/063/SRR24102363/SRR24102363_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/058/SRR24102358/SRR24102358_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/062/SRR24102362/SRR24102362_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/075/SRR28573175/SRR28573175_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/061/SRR24102361/SRR24102361_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/060/SRR24102360/SRR24102360_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/077/SRR28573177/SRR28573177_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/076/SRR28573176/SRR28573176_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/059/SRR24102359/SRR24102359_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/075/SRR28573175/SRR28573175_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/061/SRR24102361/SRR24102361_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR241/062/SRR24102362/SRR24102362_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR285/076/SRR28573176/SRR28573176_2.fastq.gzprimaryOK200GenomicsBiomedical chemistry, School of Molecular and Cellular Biology, Graduate School of Medicine, The University of Tokyohttps://www.ebi.ac.uk/ena/browser/view/PRJNA953347Entamoeba histolyticaTo know the role of Rho small GTPases of enteric protozoan parasite Entamoeba histolytica, ten Rho small GTPases were specifically silenced and examined the effect on virulence-related phenotypes. Among them, EhRacM gene silence caused an enhancement in macropinocytosis. To clarify transcriptomic difference in EhRacM gene silenceing strain, RNA-seq analysis was conducted. Overall design: To know the role of Rho small GTPases of enteric protozoan parasite Entamoeba histolytica, ten Rho small GTPases were specifically silenced and examined the effect on virulence-related phenotypes. Among them, EhracM gene silencing caused an enhancement in macropinocytosis. Also, EhracJ gene silencing caused a slight decrease in trogocytosis. To clarify the transcriptomic differences in EhracM gene silenced strain and EhracJ gene silenced strain, RNA-seq analysis was conducted.xref:PubMed:39536056pathogen:protozoanpathogenprotozoaENAstrain, epigenetic, gene silencing, Inactivation, Gene Inactivation, Strain, Sprain, Whole Transcriptome Shotgun Sequencing, Strain., RNA-seq, inhibition of gene expression, Gene, cultivar, Strains, Sprains, Strains and Sprains, Silencing, ecotypestrain, epigenetic, gene silencing, Inactivation, Gene Inactivation, Strain, Sprain, Whole Transcriptome Shotgun Sequencing, Strain., RNA-seq, inhibition of gene expression, Gene, cultivar, Strains, Sprains, Strains and Sprains, Silencing, ecotypefalseThe results of RNA-seq for EhracM gene silencing strain and EhracJ gene silencing strainThe results of RNA-seq for EhracM gene silencing strain and EhracJ gene silencing strain2025-09-242024-06-22PRJNA953347GSE229171575939536056