ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR322/071/SRR32263971/SRR32263971.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/062/SRR27961162/SRR27961162.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/077/SRR32177877/SRR32177877.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/065/SRR26156265/SRR26156265.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/094/SRR24451094/SRR24451094.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/099/SRR24451099/SRR24451099.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/034/SRR24288734/SRR24288734.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/047/SRR32194847/SRR32194847_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/002/SRR24451102/SRR24451102.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/052/SRR26156252/SRR26156252.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/035/SRR32194835/SRR32194835.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/060/SRR26156260/SRR26156260.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/048/SRR32194848/SRR32194848_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/086/SRR32177886/SRR32177886.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/039/SRR24288739/SRR24288739.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/039/SRR32194839/SRR32194839.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/034/SRR32194834/SRR32194834.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/056/SRR26156256/SRR26156256.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/085/SRR32177885/SRR32177885.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/043/SRR24288743/SRR24288743.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/078/SRR26156278/SRR26156278.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/070/SRR27961170/SRR27961170.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/061/SRR26156261/SRR26156261.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/003/SRR24451103/SRR24451103.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/079/SRR32173579/SRR32173579.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/093/SRR24451093/SRR24451093.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/030/SRR24288730/SRR24288730.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/046/SRR32194846/SRR32194846_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/076/SRR32177876/SRR32177876.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/069/SRR26156269/SRR26156269.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/037/SRR24495237/SRR24495237.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/029/SRR24288729/SRR24288729.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/070/SRR26156270/SRR26156270.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/081/SRR32173581/SRR32173581.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/036/SRR32194836/SRR32194836.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/063/SRR27961163/SRR27961163.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/001/SRR24451101/SRR24451101.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/090/SRR24451090/SRR24451090.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/049/SRR26156249/SRR26156249.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/061/SRR27961161/SRR27961161.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/072/SRR26156272/SRR26156272.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/050/SRR32194850/SRR32194850.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/090/SRR32177890/SRR32177890.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/027/SRR24288727/SRR24288727.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/035/SRR24288735/SRR24288735.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/064/SRR26156264/SRR26156264.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/082/SRR32177882/SRR32177882.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/084/SRR32173584/SRR32173584.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/075/SRR26156275/SRR26156275.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/051/SRR32194851/SRR32194851.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/080/SRR32177880/SRR32177880.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/026/SRR24288726/SRR24288726.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/032/SRR24288732/SRR24288732.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/067/SRR26156267/SRR26156267.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/074/SRR32177874/SRR32177874.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/057/SRR26156257/SRR26156257.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/042/SRR24288742/SRR24288742.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/083/SRR32173583/SRR32173583.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/081/SRR32177881/SRR32177881.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/074/SRR26156274/SRR26156274.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/049/SRR32194849/SRR32194849_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/033/SRR24288733/SRR24288733.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/082/SRR32173582/SRR32173582.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/073/SRR32177873/SRR32177873.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/038/SRR32194838/SRR32194838.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/066/SRR26156266/SRR26156266.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/041/SRR24288741/SRR24288741.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/073/SRR26156273/SRR26156273.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/058/SRR26156258/SRR26156258.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/060/SRR27961160/SRR27961160.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/059/SRR26156259/SRR26156259.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/088/SRR32177888/SRR32177888.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/076/SRR26156276/SRR26156276.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/037/SRR32194837/SRR32194837.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/000/SRR24451100/SRR24451100.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/054/SRR26156254/SRR26156254.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/028/SRR24288728/SRR24288728.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/080/SRR32173580/SRR32173580.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/045/SRR24288745/SRR24288745.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/038/SRR24495238/SRR24495238.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/088/SRR24451088/SRR24451088.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/083/SRR32177883/SRR32177883.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/040/SRR24288740/SRR24288740.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/046/SRR32194846/SRR32194846_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/047/SRR32194847/SRR32194847_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/085/SRR32173585/SRR32173585.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/069/SRR27961169/SRR27961169.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/075/SRR32177875/SRR32177875.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/032/SRR32194832/SRR32194832.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/091/SRR24451091/SRR24451091.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/071/SRR26156271/SRR26156271.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/062/SRR26156262/SRR26156262.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/037/SRR24288737/SRR24288737.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/048/SRR32194848/SRR32194848_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/065/SRR27961165/SRR27961165.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/092/SRR24451092/SRR24451092.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/091/SRR32177891/SRR32177891.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/031/SRR24288731/SRR24288731.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/068/SRR26156268/SRR26156268.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/033/SRR32194833/SRR32194833.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/036/SRR24288736/SRR24288736.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/063/SRR26156263/SRR26156263.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/084/SRR32177884/SRR32177884.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/055/SRR26156255/SRR26156255.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/064/SRR27961164/SRR27961164.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/077/SRR26156277/SRR26156277.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/044/SRR24288744/SRR24288744.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/049/SRR32194849/SRR32194849_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/086/SRR32173586/SRR32173586.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/089/SRR24451089/SRR24451089.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/053/SRR26156253/SRR26156253.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/095/SRR24451095/SRR24451095.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/031/SRR32194831/SRR32194831.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/068/SRR27961168/SRR27961168.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/066/SRR27961166/SRR27961166.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/038/SRR24288738/SRR24288738.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/046/SRR24288746/SRR24288746.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/089/SRR32177889/SRR32177889.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR322/070/SRR32263970/SRR32263970.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/078/SRR32177878/SRR32177878.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/098/SRR24451098/SRR24451098.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/097/SRR24451097/SRR24451097.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/059/SRR27961159/SRR27961159.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/051/SRR26156251/SRR26156251.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/079/SRR26156279/SRR26156279.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/049/SRR24288749/SRR24288749.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR261/050/SRR26156250/SRR26156250.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR242/047/SRR24288747/SRR24288747.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/079/SRR32177879/SRR32177879.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/030/SRR32194830/SRR32194830.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR244/096/SRR24451096/SRR24451096.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR321/087/SRR32177887/SRR32177887.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/067/SRR27961167/SRR27961167.fastq.gzprimaryOK200GenomicsUNC Chapel Hillhttps://www.ebi.ac.uk/ena/browser/view/PRJNA961141Mus musculusxref:PubMed:40118040The mechanisms and biological roles of Polycomb repressive complex (PRC) recruitment by lncRNAs remain unclear. To gain insight, we expressed two lncRNAs that recruit PRCs to multi-megabase domains, Airn and Xist, from an ectopic locus and compared effects. Unexpectedly, ectopic Airn recruited PRC1 and PRC2 to chromatin with a potency resembling Xist yet did not repress genes. Compared to PRC2, PRC1 was more proximal to Airn and Xist, where its enrichment over C-rich elements required the RNA-binding protein HNRNPK. Fusing Airn to Repeat A, the domain required for gene silencing by Xist, enabled gene silencing and altered local patterns but not relative levels of PRC-directed modifications. Our data suggest that endogenously, Airn recruits PRCs to maintain rather than initiate gene silencing that PRC recruitment occurs independently of Xist Repeat A and that protein-bridged interactions, not direct RNA contacts, underlie PRC recruitment by Airn, Xist, and other lncRNAs. Overall design: Chromatin Immunoprecipitation DNA (ChIP-) and total RNA sequencing (RNA-Seq) in RMCE ESCs and TSCs. RNA immunoprecipitation-sequencing (RIP-Seq) in RMCE ESCs. Chromatin immunoprecipitation DNA-sequencing (ChIP-Seq) in RMCE ESCs. Total RNA sequencing (RNA-Seq) in RMCE ESCs and TSCs. Nuclear, chromatin, and Cytoplasm RNA-seq from Cellular fractionation of TSCs. RNA immunoprecipitation-sequencing (RIP-Seq) in TSCs.ENAfalseIsogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAsIsogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAs2025-09-242025-02-22PRJNA961141GSE2304421009040118040