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Center for Cancer Research, University of Gothenburg"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA962554"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:38671504"],"long_description":["Myxoid liposarcoma (MLS) is the second most common type of liposarcoma and is characterized by the fusion oncogene FUS‐DDIT3 or the less common EWSR1‐DDIT3. FUS-DDIT3 is causative in tumor development, but the molecular function of FUS-DDIT3 remains largely unknown. In addition, the tumor microenvironment is important in MLS development. However, due to a lack of relevant experimental model systems, it has been challenging to examine the microenvironmental impact in MLS development. Therefore, we have developed an in vivo-like experimental model system utilizing cell-free scaffolds derived from myxoid liposarcoma patient-derived xenograft tumors that can be repopulated with tumor cells. To study the effect of FUS-DDIT3 expression in combination with the MLS microenvironment, we analyzed MLS cell lines as well as fibrosarcoma cells with and without ectopic FUS-DDIT3 expression cultured in scaffolds using cells cultured in monolayers as reference. We identified several gene networks and processes that are uniquely associated with FUS-DDIT3 expression as well as microenvironment. The use of in vivo-like experimental systems opens new possibilities to understand tumor development and develop treatments. Overall design: Five cell lines were cultured in scaffolds and monolayer (2D) in 3-5 replicates and analyzed using RNA sequencing: Two MLS cell lines, 1765-92 and 2645-94, as well as HT1080 fibrosarcoma cells with and without fusion oncogene FUS-DDIT3 including native HT1080 cells, HT1080 cells expressing EGFP and HT1080 cells expressing FUS-DDIT3-EGFP."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression profiles associated with myxoid liposarcoma scaffold culture compared to monolayer growth and effect of fusion oncogene FUS-DDIT3","description":"Gene expression profiles associated with myxoid liposarcoma scaffold culture compared to monolayer growth and effect of fusion oncogene FUS-DDIT3","dates":{"last_updated":"2025-09-24","first_public":"2024-01-02"},"accession":"PRJNA962554","cross_references":{"GEO":["GSE230773"],"taxon":["9606"],"PubMed":["38671504"]}}