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musculus"],"tag":["xref:PubMed:37365386"],"long_description":["Following infection or vaccination, activated B cells at extrafollicular sites or within germinal centers (GCs) undergo vigorous clonal proliferation. Proliferating lymphocytes have been shown to undertake lactate dehydrogenase A (LDHA)-dependent aerobic glycolysis however, the specific role of this metabolic pathway in a B cell transitioning from a naïve to a highly proliferative, activated state remains poorly defined. Here, we deleted LDHA in a stage- and cell-specific manner. We find that ablation of LDHA in a naïve B cell did not profoundly affect its ability to undergo a T cell-independent extrafollicular B cell response. On the other hand, LDHA-deleted naïve B cells had a severe defect in the capacities to form GCs and mount GC-dependent antibody responses. In addition, loss of LDHA in T cells severely compromised B cell-dependent immune responses. Strikingly, when LDHA was deleted in activated, as opposed to naïve, B cells, there were only minimal effects on the GC reaction and in the generation of high-affinity antibodies. These findings strongly suggest that naïve and activated B cells have distinct metabolic requirements that are further regulated by niche and cellular interactions. Overall design: JH4 sequencing of LDHA-sufficient and -deficient splenocytes"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput JH4 sequencing of germinal centers of LDHA-suffiicient and -deficient B cells","description":"High-throughput JH4 sequencing of germinal centers of LDHA-suffiicient and -deficient B cells","dates":{"last_updated":"2025-09-24","first_public":"2023-06-03"},"accession":"PRJNA973112","cross_references":{"GEO":["GSE232658"],"taxon":["10090"],"PubMed":["37365386"]}}