{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Tsinghua university"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA977471"],"long_description":["The ability to detect nucleic acids with high sensitivity and specificity is fundamental for monitoring infectious disease and tracing their spread across populations and environment. Among available detection methods, reverse transcription polymerase chain reaction (RT-qPCR) stands as the gold standard due to its extraordinary sensitivity, which stems from its ability to exponentially amplify unique sequences in target RNA. Recently, a promising alternative method for nucleic acid detection has emerged through the development of a CRISPR Cas13-based method. This technique capitalizes on the CRISPR RNA (crRNA)-dependent activation of Cas13 nuclease, inducing trans-cleavage and release of caged reporter molecules, which can be quantified using a fluorescence detector. Cas13 detection can differentiate single-base mismatches and reach attomolar sensitivity when coupled with pre-amplification. Continuous advancement in Cas13 systems has demonstrated detection even without pre-amplification, exemplified by the potent Leptotrichia buccalis Cas13 homolog (LbuCas13) with 2 crRNA, which can detect as few as 100 copies/ul of purified, intact target RNA. However, the inherent vulnerability of RNA to degradation poses a significant challenge. RNA degradation is a universal issue that can occur during sample collection, transportation, storage, and handling, and can be triggered by a wide array of factors, including heat, pH changes, and chemical agent. This degradation severely affects the detection accuracy, greatly increasing the risks of false-negative results. To date, no current method has successfully overcome the grand challenge of detecting highly degraded RNA. To address this unmet need, we developed a technique, Cas13-TRACER, specifically designed for the detection of severely degraded RNA."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"Cas13-TRACER enables detection of severely degraded RNA","dates":{"last_updated":"2024-05-11","first_public":"2024-05-11"},"accession":"PRJNA977471","cross_references":{}}