ENAGenomicsPharmacology, Kyoto University Graduate School of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA99341Mus musculusInactivation of TGF-beta family signaling is implicated in colorectal tumor progression. Using the cis-Apc/Smad4 mutant mice, a model of invasive colorectal cancer whose TGF-beta family signaling is blocked, we demonstrate here that a novel type of immature myeloid cells (iMCs) is recruited from the bone marrow to the tumor invasion front. These CD34+ iMCs express MMP9/2 and CC-chemokine receptor 1 (CCR1), and migrate toward its ligand CCL9. In the adenocarcinomas, expression of CCL9 is increased in the tumor epithelium. Such changes in the chemokine expression or the CD34+ iMC recruitment are not observed in the Apc (+/–) mice, a model of adenomatous polyposis. By knocking out Ccr1 gene in the cis-Apc/Smad4 mutant mice, we further demonstrate that lack of CCR1 prevents the accumulation of CD34+ iMCs at the invasion front and suppresses tumor invasion. These results indicate that loss of the TGF-beta family signaling in tumor epithelium causes accumulation of iMCs that help tumor invasion. Keywords: disease state analysis Overall design: We compared the gene expression profile of the cis-Apc/Smad4 adenocarcinomas with that of the Apc (+/–) adenomas to look for chemokines that were increased in the adenocarcinomas. To this end, we used DNA Chip Consortium Mouse Microarray v2.0 (oligonucleotide microarray) that contains 65-mer oligonuleotide probes for 21,997 mouse transcripts for three individual analyses.xref:PubMed:17369830ENAAdenocarcinoma, 3.4.22.-, D-APC1, SMAD family member 4, D-APC2, D-Axin, e-apc, DmelCG1775, Adenomas, Laboratory, Daxin, Mus domesticus, jip, Oxyphilic Adenocarcinoma, apc, mini-ICE, axn, CASP-14, 2610511A05Rik, Malignant, ADNOS, House Mouse, Granular Cell Adenocarcinomas, BB234005, DP3, PPP1R46, DP2, d-axin, House, SMAD 4, l(3)SG36, Granular Cell Adenocarcinoma, DAPC, CG6193, madh4, Mus musculus domesticus, Tubular Carcinoma, Min, BTPS2, GSK3beta, Basal Cell Adenocarcinomas, CG1451, D-APC, Mice, MYHRS, Cribriform Carcinoma, dApc, dAPC, hSMAD4, MADH4, medea, XSmad4alpha, Adenoma, Swiss, Granular Cell Carcinoma, Tubular Adenocarcinoma, smad4, mAPC, Swiss Mice, Tubular Carcinomas, Cribriform Carcinomas, E(zen)3, CG7926, AI047805, SMAD4, dAPC2/E-APC, MAD homolog 4, anon-EST:Posey121, Granular Cell Carcinomas, Mothers against DPP homolog 4, cyclosome, Deletion target in pancreatic carcinoma 4 homolog, Protein G18, Smad4, DmelCG6193, BACTS2, dAxin, ahl4, DmelCG1451, dApc2, l(3)12m-137, Carcinoma, Oxyphilic, din, l(3)S044230, Basal Cell, E-APC, DP2.5, CASP14, mouse, dpc4, l(3)11m-254, Madh4, D18Wsu70e, Adenocarcinomas, d-APC, APC2, dAXIN, Apc1, APC1, xapc, Tubular, E-APC dAPC2, Malignant Adenoma, Mus, Cis, CIS, MED, apc 1, Mini-ICE, G18, apc1, axin, apc2, Granular Cell, xsmad4a, Oxyphilic Adenocarcinomas, Basal Cell Adenocarcinoma, d-APC2, dSmad4, CG1775, Mus musculus., dAPC2, dAPC1, Carcinomas, AU020952, Mus musculus, CIS-1, Caspase-14 subunit p10, Xsmad4, med, mice, CC1, 0442/30, Swiss Mouse, Cribriform, 9030605P22Rik, House Mice, Caspase-14 subunit p19, l(3)SG70, AW124434, MICE, Dm APC2, Tubular Adenocarcinomas, Malignant Adenomas, Dm APC1, DAxin, Laboratory Mice, domesticus, AW743858, Deletion target in pancreatic carcinoma 4, SOCS, GS, DmelCG7926, JIP, APC, Mouse, anaphase promoting complex, Suppressor of cytokine signaling, l(3)XIIm137, Laboratory Mouse, DPC4Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusThe intestinal adenocarcinomas of the cis-Apc/Smad4 compound mutant mice2025-09-242014-02-11PRJNA99341GSE69501009017369830