Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Tri-KIX +/- c-Myb MEFs


ABSTRACT: Transcription profiling by array of wild type and p300 KIX/KIX; CBP +/KIX primary mouse embryonic fibroblasts (MEFs) transduced with either MSCV-c-Myb_IRES-GFP or MSCV-IRES-GFP retrovirus to determine the effect of mutating the KIX domain of CBP and p300 on c-Myb regulated gene expression. CBP KIX mutation (MGI:3578129) and p300 KIX mutation (MGI:3578128).

ORGANISM(S): Mus musculus

SUBMITTER: Lawryn Kasper 

PROVIDER: E-MTAB-1973 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Genetic interaction between mutations in c-Myb and the KIX domains of CBP and p300 affects multiple blood cell lineages and influences both gene activation and repression.

Kasper Lawryn H LH   Fukuyama Tomofusa T   Lerach Stephanie S   Chang Yunchao Y   Xu Wu W   Wu Song S   Boyd Kelli L KL   Brindle Paul K PK  

PloS one 20131210 12


Adult blood cell production or definitive hematopoiesis requires the transcription factor c-Myb. The closely related KAT3 histone acetyltransferases CBP (CREBBP) and p300 (EP300) bind c-Myb through their KIX domains and mice homozygous for a p300 KIX domain mutation exhibit multiple blood defects. Perplexingly, mice homozygous for the same KIX domain mutation in CBP have normal blood. Here we test the hypothesis that the CBP KIX domain contributes subordinately to hematopoiesis via a genetic int  ...[more]

Similar Datasets

2014-02-10 | E-MTAB-1974 | biostudies-arrayexpress
2012-12-31 | E-GEOD-34224 | biostudies-arrayexpress
2014-09-29 | E-GEOD-54452 | biostudies-arrayexpress
2008-06-16 | E-GEOD-9161 | biostudies-arrayexpress
2014-09-29 | E-GEOD-54453 | biostudies-arrayexpress
2011-05-24 | E-GEOD-29453 | biostudies-arrayexpress
2013-01-15 | E-GEOD-42551 | biostudies-arrayexpress
2018-06-10 | PXD005252 | Pride
2013-01-15 | E-GEOD-38110 | biostudies-arrayexpress
2014-04-06 | E-GEOD-45834 | biostudies-arrayexpress