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Mouse Stereocilia Capping Protein Immunoaffinity Purification

ABSTRACT: Capping protein controls stereocilia length and width during hair bundle development. To determine what other proteins are involved in capping protein regulation, we carried out immunoaffinity purifications targeted at either CAPZA or CAPZB2. The starting material for immunopurification was crude stereocilia membranes isolated from mouse inner ear.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Inner Ear Vestibulum, Ear Hair Cell, Cochlea, Auditory Hair Cell, Inner Ear, Vestibular Hair Cell

SUBMITTER: Peter Barr-Gillespie

LAB HEAD: Peter Barr-Gillespie

PROVIDER: PXD006077 | Pride | 2017-08-25

REPOSITORIES: Pride

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Heterodimeric capping protein is required for stereocilia length and width regulation.

Avenarius Matthew R MR Krey Jocelyn F JF Dumont Rachel A RA Morgan Clive P CP Benson Connor B CB Vijayakumar Sarath S Cunningham Christopher L CL Scheffer Deborah I DI Corey David P DP Müller Ulrich U Jones Sherri M SM Barr-Gillespie Peter G PG

The Journal of cell biology 20170912 11

Control of the dimensions of actin-rich processes like filopodia, lamellipodia, microvilli, and stereocilia requires the coordinated activity of many proteins. Each of these actin structures relies on heterodimeric capping protein (CAPZ), which blocks actin polymerization at barbed ends. Because dimension control of the inner ear's stereocilia is particularly precise, we studied the CAPZB subunit in hair cells. CAPZB, present at ∼100 copies per stereocilium, concentrated at stereocilia tips as h ...[more]

PMID: 28899994

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