Dataset Information

Global acetylation profiling for multiple Arabidopsis thaliana plastid N-terminal acetyltransferases

ABSTRACT: Protein acetylation is a universally conserved modification occurring on N-termini (N-Terminal acetylation, NTA). Although recent reports indicate that NTA occur frequently in plant plastids, little is known about the machinery involved in plastid acetylation and why these modifications are that frequent in this organelle. Searches for new putative N-acetyltransferase genes in Arabidopsis thaliana highlighted eight putative candidates located at plastid subcellular location. In this study, we investigated the N-acetyltransferase specificity of these enzymes using the global acetylome profiling test (GAP test).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli

SUBMITTER: Willy Bienvenut

LAB HEAD: Willy Bienvenut

PROVIDER: PXD016205 | Pride | 2020-07-13

REPOSITORIES: Pride

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Publications

Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation.

Bienvenut Willy V WV Brünje Annika A Boyer Jean-Baptiste JB Mühlenbeck Jens S JS Bernal Gautier G Lassowskat Ines I Dian Cyril C Linster Eric E Dinh Trinh V TV Koskela Minna M MM Jung Vincent V Seidel Julian J Schyrba Laura K LK Ivanauskaite Aiste A Eirich Jürgen J Hell Rüdiger R Schwarzer Dirk D Mulo Paula P Wirtz Markus M Meinnel Thierry T Giglione Carmela C Finkemeier Iris I

Molecular systems biology 20200701 7

Protein acetylation is a highly frequent protein modification. However, comparatively little is known about its enzymatic machinery. N-α-acetylation (NTA) and ε-lysine acetylation (KA) are known to be catalyzed by distinct families of enzymes (NATs and KATs, respectively), although the possibility that the same GCN5-related N-acetyltransferase (GNAT) can perform both functions has been debated. Here, we discovered a new family of plastid-localized GNATs, which possess a dual specificity. All cha ...[more]

PMID: 32633465

Dataset Information

Global acetylation profiling for multiple Arabidopsis thaliana plastid N-terminal acetyltransferases

Publications

Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation.

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