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AtNAA50 N-terminal acetylation profiling using A. thaliana naa50 KO mutants

ABSTRACT: N-terminal acetylation is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-terminal acetyltransferases. AtNAA50 interreact with the NatA complex (NAA10 and NAA15) to form the NatE complex. In this study, we investigated the Arabidopsis thaliana N-terminal acetylome in leaf cells with KO naa50 gene to identify in vivo specific substrates using the SILProNAQ approach.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Willy Bienvenut

LAB HEAD: Willy Bienvenut

PROVIDER: PXD017770 | Pride | 2020-07-15

REPOSITORIES: Pride

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NAA50 Is an Enzymatically Active N α-Acetyltransferase That Is Crucial for Development and Regulation of Stress Responses.

Armbruster Laura L Linster Eric E Boyer Jean-Baptiste JB Brünje Annika A Eirich Jürgen J Stephan Iwona I Bienvenut Willy V WV Weidenhausen Jonas J Meinnel Thierry T Hell Ruediger R Sinning Irmgard I Finkemeier Iris I Giglione Carmela C Wirtz Markus M

Plant physiology 20200527 4

Nα-terminal acetylation (NTA) is a prevalent protein modification in eukaryotes. In plants, the biological function of NTA remains enigmatic. The dominant N-acetyltransferase (Nat) in Arabidopsis (Arabidopsis thaliana) is NatA, which cotranslationally catalyzes acetylation of ∼40% of the proteome. The core NatA complex consists of the catalytic subunit NAA10 and the ribosome-anchoring subunit NAA15. In human (Homo sapiens), fruit fly (Drosophila melanogaster</i ...[more]

PMID: 32461302

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