Integration of genome-wide expression profiling with linkage analysis is a new approach to identifying genes underlying complex traits. We applied this approach to the regulation of gene expression in the BXH/HXB panel of rat recombinant inbred strains, one of the largest available rodent recombinant inbred panels and a leading resource for genetic analysis of the highly prevalent metabolic syndrome. In two tissues important to the pathogenesis of the metabolic syndrome, we mapped cis- and trans ...[more]
Project description:Mononuclear cells were isolated from granulocyte colony stimulating factor mobilised peripheral blood. CD34+ cells were selected and separated into adherent and non-adherent cells. Adherent and non-adherent samples were amplified and labelled using two IVT cycles, and gene expression profiling was performed by hybridisation to Affymetrix Human Genome U133 Plus2.0 Arrays.
Project description:A mouse leydig tumour cell line (mLTC-1) was grown in culture and stimulated with either human chorionic gonadotropin (hCG, 10 ng/ml) alone or hCG & bisphenol A (BPA, an endrocrine disrupting chemical, 10 uM) for three hours. Cells from triplicate plates were harvested, RNA extracted, labelled and hybridised to Affymetrix Mouse genome 430 2.0 arrays. Preliminary work by RT-PCR had shown that stimulation of the cell line by BPA resulted in changes in gene expression in the spermatogenesis pathway at this time point.
Project description:Undifferentiated mouse embryonic stem cells stably expressing the HOXB1 transcription factor under doxycycline tet-on control were differentiated into embryoid bodies. Nestin+ neuroepithelial cells were selected by transfer of the embryoid bodies to ISTFn media. Expression of HOXB1 was induced by addition of doxycycline or not induced on the seventh day of selection and also for one day after trypsination and transfer to laminin/polyornithine substrate on matrigel substrate.
Project description:Title: Gene Expression analysis of the role of RIP140 in adipocyte differentiation<br/> Description: RIPKO cells are mouse embryo fibroblasts (MEF) that were derived from <br/> RIP140-knockout mice and have been established as a RIP140 null cell line,<br/> while RIPKO-L7 and RIPKO-L16 are RIPKO cells for which the expression of<br/> RIP140 has been reintroduced using a lentiviral expression system. <br/> Using an established standard cocktail of hormones that includes IBMX, <br/> insulin, dexamethasone and a PPAR gamma agonist the cells were <br/> differentiated into adipocytes and RNA isolated at day 0 and day 10 <br/> after the addition of the cocktail.
Project description:Title: Comparison of ovarian gene expression in RIP140 wild type, heterozygous and knock out mice.<br/> Description: RIP140 null mice fail to ovulate. This experiment was designed to <br/> determine the gene expression profile of these animals compared <br/> to wild type and heterozygous animals following hormone treatments <br/> that induce the biological changes required for ovulation to occur.
Project description:Ventricles were removed from 2-day Sprague_Dawley rat litters from which primary cardiac myocytes (>95%) were isolated. Myocytes were either untreated or exposed to either endothelin-1 for 30, 60 or 120 minutes, cycloheximide for 70 or 130 minutes, endothelin-1 for 10 minutes followed by cycloheximide for 70 minutes, azekenpaullone for 60 minutes before extraction of total or polysomal (actively transcribed) RNA. Labelled cRNA was hybridised to Affymetrix Rat 230 2.0 arrays.
Project description:Four male SHR/Ola, BN and SHR-18 rats were fed a normal diet and sacrificed at 9 weeks of age. Four male SHR/Ola and SHR-18 rats at 8 weeks of age were fed 1% NaCl for one week and then sacrificed. Kidneys were removed and frozen in liquid nitrogen for all 20 animals. Total RNA was isolated, labelled cRNA was generated and hybridised to Affymetrix Rat RG-U34ABC arrays.
Project description:Title: Gene expression analysis of indolent and aggressive forms of Chronic Myeloid Leukaemia (CML). Description: Chronic Myeloid Leukaemia presents in chronic phase (CP) and terminates in 'blast crisis'. Despite a common abnormality, the duration of CP is variable. The aim is to compare the gene expression profiles of the indolent and aggressive forms of CML. All samples were taken within 3 months of first diagnosis. Indolent patients were defined by chronic phase CML, duration minimum 7 years. Aggressive patients were defined by chronic phase, duration maximum 3 years.
Project description:Gene transcription profiling in Epidydimal fat pad (EPD) following High fat versus normal diet in C57/Bl6 mice. Characterisation of molecular mechanisms of in vivo insulin action in mouse models of experimentally induced Insulin resistance. Switched the diet when mice were 5 weeks old, then sampled at 8 days.
Project description:Gene transcription profiling in muscle following muscle versus normal diet in C57/Bl6 mice. Switched the diet when mice were 5 weeks old, then sampled at 8 days, 3 weeks and 15 weeks.