Transcriptomics

Dataset Information

350

Comparative genomic hybridization by array of Streptococcus pneumoniae isolates from the Mitis group to identify pneumococcal virulance genes


ABSTRACT: Seven streptococcal isolates from the Mitis group were analysed for the presence of pneumococcal gene homologues using comparative genomic hybridization studies with microarrays based on open reading frames from the genomes of Streptococcus pneumoniae TIGR4 and R6. The diversity of pneumolysin (ply) and neuramindase A (nanA) gene sequences was explored in more detail in a collection of 14 S. pseudopneumoniae and 29 Mitis group isolates respectively. The Mitis group isolates used in the microarray experiments included a type strain (NCTC 12261), 2 S. mitis isolates from the nasopharynx of children, one S.mitis from infective endocarditis, one S. mitis isolate from a dental abscess, plus one S. oralis isolate and one S. pseudopneumoniae isolate from the nasopharynx of children. The results of the microarray study showed that the 5 S. mitis isolates had homologues to between 67-82% of pneumococcal virulence genes, S. oralis hybridised to 83% and S. pseudopneumoniae to 92% of identified pneumococcal virulence genes. Comparison of the pneumolysin, mitilysin (mly) and newly identified pseudopneumolysin (pply) gene sequences revealed that mly and pply genes are more closely related to each other than either is to ply. In contrast, the nanA gene sequences in the pneumococcus and streptococci from the Mitis group are closely clustered together sharing 99.4-99.7% sequence identity with pneumococcal nanA alleles. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-82

INSTRUMENT(S): NanoDrop

ORGANISM(S): Streptococcus pneumoniae  

SUBMITTER: BuG@S group  

PROVIDER: E-BUGS-82 | ArrayExpress | 2010-07-22

REPOSITORIES: ArrayExpress

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Publications

Detection of large numbers of pneumococcal virulence genes in streptococci of the mitis group.

Johnston Calum C   Hinds Jason J   Smith Andrew A   van der Linden Mark M   Van Eldere Johan J   Mitchell Tim J TJ  

Journal of clinical microbiology 20100602 8


Seven streptococcal isolates from the mitis group were analyzed for the presence of pneumococcal gene homologues by comparative genomic hybridization studies with microarrays based on open reading frames from the genomes of Streptococcus pneumoniae TIGR4 and R6. The diversity of pneumolysin (ply) and neuraminidase A (nanA) gene sequences was explored in more detail in a collection of 14 S. pseudopneumoniae and 29 mitis group isolates, respectively. The mitis group isolates used in the microarray  ...[more]

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