Project description:Arabidopsis thaliana Col-0 wildtype plants were grown on soil (Jiffy7 peat pellets 42 mm) to the respective developmental stage (1.04 or 1.08 Boyes scale). The plants were sprayed with 50 µM Abscisic acid and incubated for 15 min or 2 hours under normal growth conditions. Leaves 1 and 2 were harvested and immediately frozen in liquid nitrogen.
Project description:In-vitro-cultured seedlings of WT (Col-0) and all possible combinations of single, double, and triple T-DNA insertion mutants of the cytokinin receptors AHK2 (ahk2-5), AHK3 (ahk3-7) and AHK4 (cre1-2) at growth stage 1.00 were treated for 0, 15 and 120 min with 5micromolar of the synthetic cytokinin 6-benzyladednie (BA).
Project description:A dye swap experiment to compare Arabidopsis thaliana ecotype Col-0 grown to stage 1.04 (Boyes scale) at VIB, Plant systems biology, Gent, Belgium with Arabidopsis thaliana ecotype Col-0 grown to stage 1.04 (Boyes scale) at FU Berlin.
Project description:Dye swap experiment comparing Arabidopsis thaliana Col-0 seedlings at growth stage 1.04 grown at VIB, Plant systems biology, Gent, Belgium against a sample meeting the same experimental conditions grown at FU Berlin.
Project description:A large-scale time course expression profiling of wild type (Mla12/Rar1/Rom1) and mutants (mla12-M66, M82 (rar1-1), M100 (rar1-2) and rom1) of barley cultivar Sultan 5 was conducted to understand the molecular mechanisms of delayed powdery mildew resistance. Barley plants were inoculated with powdery mildew pathogen isolate 5874. First leaves of inoculated and non-inoculated plants were harvested at six time points after pathogen inoculation. The experiment was laid out in split-split-plot design with 180 experimental units (3 replications x 2 treatments (inoculated and non-inoculated) x 5 genotypes x 6 time points).
Project description:A parallel expression profiling of wild-type and loss-of-function mutants of Mla6 and Mla1 powdery mildew resistance alleles was conducted using Barley1 GeneChip. Barley plants were inoculated with powdery mildew isolate 5874 and first leaves were harvested at 6 time points after pathogen inoculation. This experiment was conducted in split-split-plot experimental design with 3 replications.
Project description:Genome-wide transcription profiles analysis of the heat-shocked wild-type strain under moderate (40¡C) and severe heat stress (50¡C) revealed that a large number of genes are differentially expressed after heat shock. 358 genes were up-regulated and 420 were down-regulated in response to moderate heat shock 35 (40¡C) in C. glutamicum. Our results confirmed the HrcA/CIRCE-dependent and HspR/HAIR-dependent up-regulation of chaperones following heat shock. Other genes including COG groups related to macromolecule biosynthesis and several transcriptional regulators (COG class K) were up-regulated, explaining the large number of genes affected by heat shock. Mutants having deletions in the hrcA or hspR regulators were constructed, which allowed the complete identification of the genes controlled by those systems. The up- or down-regulation of several genes observed in the microarray experiments was validated by Northern analyses and quantitative (real-time) PCR. These analyses showed the heat shock intensity-dependent response (differential response) presented by the HspR/HAIR system, in contrast with the non-differential response shown by the HrcA/CIRCE-regulated genes.