Transcriptomics

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Transcription profiling of rat pancreatic beta-cells in response to double stranded RNA and IFNgamma exposure


ABSTRACT: Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (polyinosinic-polycytidylic acid, PIC) in combination with IFNgamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFNgamma-effects, we determined the global profile of genes modified by these agents in primary rat beta cells. FACS-purified rat beta cells were cultured for 6 or 24 h in control condition or with IFNgamma, PIC or a combination of both agents. The gene expression profile was analyzed in duplicate with the Affymetrix RG U34A microarray.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Decio Eizirik  Junmin Liu  John Brestelli       

PROVIDER: E-CBIL-11 | ArrayExpress | 2012-02-21

REPOSITORIES: ArrayExpress

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Global profiling of double stranded RNA- and IFN-gamma-induced genes in rat pancreatic beta cells.

Rasschaert J J   Liu D D   Kutlu B B   Cardozo A K AK   Kruhøffer M M   ØRntoft T F TF   Eizirik D L DL  

Diabetologia 20031105 12


Viral infections and local production of IFN-gamma might contribute to beta-cell dysfunction/death in Type 1 Diabetes. Double stranded RNA (dsRNA) accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (tested in the form of polyinosinic-polycytidylic acid, PIC) in combination with IFN-gamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFN-gamma-effects, we determined the global profile of ge  ...[more]

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