Dataset Information


Transcription profiling of mouse brown preadipocytes from wild type and IRS knock-out cell lines

ABSTRACT: Brown preadipocytes were grown to confluence and synchronized by overnight serum starvation. Four independent RNA samples were analyzed from each IRS KO cell line and three independent clones of WT cells were separately analyzed as controls. Splitting of the samples resulted in a total 28 microarrays. 15 mg of adjusted cRNA were hybridized to Affymetrix U74A-v2 arrays.


ORGANISM(S): Mus musculus  

SUBMITTER: Junmin Liu   Ronald Kahn  

PROVIDER: E-CBIL-26 | ArrayExpress | 2007-04-06


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Prediction of preadipocyte differentiation by gene expression reveals role of insulin receptor substrates and necdin.

Tseng Yu-Hua YH   Butte Atul J AJ   Kokkotou Efi E   Yechoor Vijay K VK   Taniguchi Cullen M CM   Kriauciunas Kristina M KM   Cypess Aaron M AM   Niinobe Michio M   Yoshikawa Kazuaki K   Patti Mary Elizabeth ME   Kahn C Ronald CR  

Nature cell biology 20050515 6

The insulin/IGF-1 (insulin-like growth factor 1) signalling pathway promotes adipocyte differentiation via complex signalling networks. Here, using microarray analysis of brown preadipocytes that are derived from wild-type and insulin receptor substrate (Irs) knockout animals that exhibit progressively impaired differentiation, we define 374 genes/expressed-sequence tags whose expression in preadipocytes correlates with the ultimate ability of the cells to differentiate. Many of these genes, inc  ...[more]

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