Transcriptomics

Dataset Information

18

Translation profiling of Arabidopsis cell cultures exposed to elevated temperature and high salinity


ABSTRACT: We investigated genome-wide changes in mRNA translation in Arabidopsis thaliana suspension cell cultures exposed to brief perids of two types of stress: elevated temperature (37 degree_C) and high salinity (200 mM NaCl). To this end, we subjected polysomal RNA and non-polysomal RNA from sucrose gradient fractionated cell lysates to the co-hybridization on Agilent Arabidopsis 3 Oligo Microarrays. The ratio of signal intensities (polysomal RNA: non-polysomal RNA) was used as an indicator of the translation state for each transcript. To inspect coordination of changes in translational profiles with transcriptional profiles, we also isolated total RNAs from the same cells used for translational profiling experiments and investigated changes in accumulated transcript levels in response to each stress using the microarray. Two biological replicates were analyzed.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Hideyuki Matsuura   Ko Kato 

PROVIDER: E-DORD-69 | ArrayExpress | 2010-07-07

SECONDARY ACCESSION(S): CBX69

REPOSITORIES: ArrayExpress

altmetric image

Publications

Genome-wide analyses of early translational responses to elevated temperature and high salinity in Arabidopsis thaliana.

Matsuura Hideyuki H   Ishibashi Yu Y   Shinmyo Atsuhiko A   Kanaya Shigehiko S   Kato Ko K  

Plant & cell physiology 20100119 3


Abiotic stress influences the translation of mRNAs in plants. To gain a global view of the early translational response to abiotic stress, we investigated genome-wide changes in mRNA translation in Arabidopsis thaliana suspension cell cultures exposed to brief periods of two types of stress: elevated temperature (37 degrees C) and high salinity (200 mM NaCl). Microarray analyses revealed that polysome association of most transcripts, which were monitored by using polysomal- and non-polysomal-ass  ...[more]

Publication: 1/2

Similar Datasets

2013-12-10 | E-GEOD-52468 | ArrayExpress
2012-03-28 | E-GEOD-29744 | ArrayExpress
2015-09-01 | E-GEOD-69069 | ArrayExpress
2015-04-01 | E-GEOD-53772 | ArrayExpress
2013-03-31 | E-GEOD-38030 | ArrayExpress
2015-04-29 | E-MTAB-3537 | ArrayExpress
2014-02-01 | E-GEOD-43299 | ArrayExpress
2015-09-01 | E-GEOD-69070 | ArrayExpress
2011-07-31 | E-GEOD-27648 | ArrayExpress
2015-03-28 | E-GEOD-67356 | ArrayExpress