Transcriptomics

Dataset Information

184

Transcription profiling of human Mesenchymal Stem Cells (MSC) vs reference RNA of non-multipotent human fibroblasts HS68


ABSTRACT: Mesenchymal stem cells (MSC) resemble a multipotent adult stem cell population capable of differentiation into a number of different mesodermal cell types including adipodytes, osteoblasts, chondroblasts. Although still in debate there is some evidence, that these cells can also differentiate into cells of non-mesodermal germal layers including hepatocytes, myocytes, cardiomyocytes or neurons. Analysis of these cells in the course of differentiation makes them an intriguing model for the examination of stemness. More importantly the differentiation capacity of MSC raises high hopes for clinical applications. In this study we have isolated MSC from bone marrow under two different culture conditions, from adipose tissue and from umbilical cord blood. The genome wide gene expression profile of these different human MSC was compared with reference RNA of non-multipotent human fibroblasts HS68. The aim of this study was to elucidate common molecular characteristics of MSC as well as differences in their expression profile. These results might help for a better understanding of MSC and contribute to a reliable quality control that will be necessary for clinical applications.

INSTRUMENT(S): (Make:Axon Instruments,Model:GenePix 4000B)

ORGANISM(S): Homo sapiens  

SUBMITTER: Wolfgang Wagner  

PROVIDER: E-EMBL-4 | ArrayExpress | 2005-08-23

REPOSITORIES: ArrayExpress

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Publications

Molecular and secretory profiles of human mesenchymal stromal cells and their abilities to maintain primitive hematopoietic progenitors.

Wagner Wolfgang W   Roderburg Christoph C   Wein Frederik F   Diehlmann Anke A   Frankhauser Maria M   Schubert Ralf R   Eckstein Volker V   Ho Anthony D AD  

Stem cells (Dayton, Ohio) 20070705 10


Mesenchymal stromal cells (MSC) provide a supportive cellular microenvironment and are able to maintain the self-renewal capacity of hematopoietic progenitor cells (HPC). Isolation procedures for MSC vary extensively, and this may influence their biologic properties. In this study, we have compared human MSC isolated from bone marrow (BM) using two culture conditions, from cord blood (CB), and from adipose tissue (AT). The ability to maintain long-term culture-initiating cell frequency and a pri  ...[more]

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