Genomics

Dataset Information

7

Chromatin and DNA methylation in human neuronal cells


ABSTRACT: ett Syndrome is a neurological disorder caused by mutation in the MeCP2 gene encoding protein, which is a component of chromatin. The neurological spectrum of Rett Syndrome suggests that this protein plays an important role in the nervous system. Limitations of in vivo studies such as the accumulation of indirect effects have impeded understanding of direct consequences of the loss of MeCP2. Thus, it is important to develop simplified models of Rett Syndrome to create the disease in a dish. This can be achieved for instance by differentiating human neuronal progenitors, which carry various MeCP2 mutations, into a homogeneous neuronal population and analyze alterations in the chromatin states such as methylation, hydroxymethylation, histone modifications and changes in transcription. Protocol: LUHMES cell line of human neural progenitors was differentiated into neurons for 9 days in presence of defined medium. At the end of differentiation, neuronal genomic DNA was extracted using Qiagen DNeasy kit and bisulfite-treated. Adaptors were ligated to purified DNA and captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols. Sequencing was performed at the Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Homo sapiens  

TISSUE(S): Brain, Cultured Neuron

SUBMITTER: The Wellcome Trust Sanger Institute  

PROVIDER: E-ERAD-112 | ArrayExpress | 2013-01-31

SECONDARY ACCESSION(S): ERP001882

REPOSITORIES: ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-ERAD-112.idf.txt Idf
E-ERAD-112.sdrf.txt Txt
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