ABSTRACT: This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ Teladorsagia circumcincta, the brown stomach worm, is the most important gastro-intestinal parasite of sheep in temperate regions and is present on 100% of sheep farms in the UK. Infection with T. circumcincta causes a spectrum of disease in the host, ranging from weight loss to death, and results in significant production losses. Parasitic disease is currently controlled by treatment with anthelmintics but resistance to these drugs is widespread and threatens the viability of the livestock industry. There are now well-documented cases of UK farms harbouring triple-resistant T. circumcincta, which can no longer be controlled by any of the three major anthelmintic classes, forcing farmers to abandon sheep rearing. High-throughput sequencing of life stages of T. circumcincta will be used for gene finding and transcriptome analysis.
Project description:The aim of this study was to investigate the response in gene expression before and after exposure to the Benzimidazoles drug flubendazole in adult female Ascaridia galli worms. The nematode Ascaridia galli (order Ascaridida) is an economically important intestinal parasite responsible for increased food consumption, reduced performance, and mortality in commercial poultry production. Parasite control relies on repeated use of dewormers (anthelmintics). Benzimidazoles are currently the only anthelmintic registered against A. galli in the EU and there is an obvious risk that overuse of one drug class may lead to resistance. The worms were collected from a commercial laying hen farms before and on day three during a treatment period of 7 days with flubendazole.
Project description:Haemonchus contortus is a highly pathogenic parasitic nematode of that can infect a large number of wild and domesticated ruminant species and is the most economically important parasite of sheep and goats worldwide. Although originally a tropical parasite, it has been disseminated around the world by livestock movement and can now be found as far north as the arctic circle. Adult worms are blood feeders that reside in the abomasum (stomach) and are approximately 2cm in length when mature. They are dioecious with single females typically producing several thousand eggs per day which pass out of the host in faeces and develop to infective larvae on the pasture. H. contortus is a member of the superfamily trichostrongyloidea (Strongylida) which contains most of the economically important parasitic nematodes of grazing livestock. These parasites cost the global livestock industry billions of dollars per annum in lost production and drug costs. Resistance to all the major anthelmintic classes is now common worldwide often leading to failure of treatment and control. H. contortus is a close relative of the human hookworm species and belongs to the nearest phylogenetic group of parasites to the free-living model nematode Caenorhabditis elegans . This makes it an important model of parasitic nematode biology that is commonly used for experimental studies. The main objective of this project is to recognize genes expressed in the life stages of H. contortus.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:The aim of the current study is to identify genes that show differential expression within the gastric lymph node of parasite resistant and susceptible sheep. For this we exploited parasite-naïve Blackface lambs with diversity in their predicted genetic resistance to T. circumcincta, which were trickle-infected with L3 larvae to mimic natural infection. This regime resulted in lambs with a spectrum of resistance as assessed by adult worm counts, faecal egg counts and IgA levels. Gastric lymph node tissue was used for transcription profiling of resistant and susceptible lambs to identify genes and physiological pathways associated with the differential activation of the immune response linked to the different disease outcomes.
Project description:Liver fluke (Fasciola hepatica) infection is both a welfare and productivity issue in sheep farming. Death can result from acute infection, and deaths are becoming more frequent as anthelmintic resistance increases. New control strategies are desirable, and vaccination is a good option. Previous studies have shown an experimental vaccine based on a F. hepatica protein, cathepsin L1, (rmFhCL1) may be a viable aid to control liver fluke in cattle/sheep, however efficacy is variable. A trial was conducted on sheep immunized with rmFhCL1 following infection with F. hepatica to understand the immune response changes induced by vaccinination at a molecular level . Peripheral blood mononuclear cells (PBMCs) were isolated at four different time points for RNA-Seq analysis. Genes differentially expressed between vaccinated and control animals were identified. Their functional roles were studied using in silico methods. Overall design: Four vaccinated and five unvaccinated (control) lambs were used in this study. Blood was collected for all animals at pre-vaccination (Time point 0), pre-infection (Time point 1), one week post infection (Time point 2) and 14 weeks post infection (Time point 3). PBMCs were isolated and RNA extracted. A total of 36 libraries were constructed and sequenced using the Illumina Hiseq 2500 platform.
Project description:Microbiome DNA from the adhering fraction of a sheep rumen. The RSTs were generated using an improved version of SARST (referred to as iSARST) from the microbiome DNA extracted from the adhering fraction of the rumen content taken from a sheep. The iSARST method is going to be submitted to Nature Biotechnology for publication. Keywords: other
Project description:Gastrointestinal nematode (GIN) is a major economic and health concern is sheep farming. Sheep breeds such as Texel are relatively resistant to GIN than the Suffolk. With the objective to understand the underlying genetic mechanism of resistance and susceptibility at the transcriptomic level, two groups of animal from both the breed were artificially (orally) infected with 30,000 L3 larvae of prominent GIN Teladorsagia circumcincta. Subgroups of animals from each breed were slaughtered on day 0, 3, 7, 14 and 21 of post infection (p.i.). Transcriptomic profiling of abomasal lymph node was performed using RNA-seq. The perturbations in gene expression profiles in both the breeds were evident and Texel showed a more tightly regulated immune response than the Suffolk. The number of differentially expressed (DE) genes between the breeds was highest (437) on un-infected control (day 0) and lowest (173) on day 7 p.i.. Pathway analysis of DE genes identified 3 significant pathways, which involved only more highly expressed genes of Suffolk breed on day 0 and only more highly expressed genes of Texel (with one exception) on day 7 p.i.. The Th1, Th2 and Treg response was evident in response to GIN in Texel and was synchronized, while in Suffolk Th1 response was reduced after infection and pronounced Th2 and Treg was not evident. The study suggests maximum level of transcriptional activity in both breeds on day 7 p.i. and there was a shift of transcriptional activity from Suffolk on day 0 to Texel on day 7 p.i.. Suffolk had a reduced Th1 response with less pronounced Th2 and Treg immune response, while Texel had an active and synchronized Th1/Th2/Treg immune response in response to GIN infection. Abomasal lymph node tissue was taken from control (n=10) and experimentally infected (with T. circumcincta) lambs (n=36) from Texel and Suffolk breed on day 0, 3, 7, 14 and 21 post infection.
Project description:Odom_mouse_C57BL6J_input This experiment provides chromatin immunoprecipitation input sequences (i.e. no antibody was added during the ChIP protocol), suitable for use as input for ChIP experiments on C57BJ/6J mice. ArrayExpress Release Date: 2011-03-01 Publication Title: Pol III occupancy of tRNA genes is highly divergent between loci but highly conserved by amino acid isotypes Publication Author List: Claudia Kutter; Gordon D. Brown; Stephen Watt; Michael D. Wilson; Angela Goncalves; Robert J. White, Duncan T. Odom Person Roles: submitter Person Last Name: Brown Person First Name: Gordon Person Mid Initials: D Person Email: email@example.com Person Phone: +44(0)1223404275 Person Address: Cancer Research UK, Robinson Way, Cambridge, CB2 0RE, UK Person Affiliation: CRUK-CRI Overall design: Experimental Design: species_design Experimental Design: replicate_design Experimental Factor Name: SPECIES Experimental Factor Name: TISSUE Experimental Factor Name: ChIP Experimental Factor Type: organism Experimental Factor Type: organism_part Experimental Factor Type: immunoprecipitate Quality Control Type: biological_replicate
Project description:Blackface Teladorsagia Solexa Analysis of resistance to Teladosagia ArrayExpress Release Date: 2010-12-08 Publication Author List: Pemberton, J.; Beraldi, D.; Craig, B. and Hopkins, J. Publication Title: Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs Publication Author List: Sylvain Aubry, Mali Salmon, Kim M Rutherford, Paul Bertone, Andrea Brautigam, Andreas PM Weber, Krystyna A Kelly, Julian M Hibberd Publication Title: De novo transcriptome assembly enables quantitative expression analysis in non-sequenced model organisms Person Roles: submitter Person Last Name: Hopkins Person First Name: John Person Mid Initials: Person Email: firstname.lastname@example.org Person Phone: 44 131 650 6160 Person Address: Roslin Institute, Summerhall, Edinburgh. EH9 1QH. UK Person Affiliation: University of Edinburgh Overall design: Experimental Design: growth_condition_design Experimental Design: co-expression_design Experimental Factor Name: CLINICALINFORMATION Experimental Factor Name: GROWTHCONDITION Experimental Factor Type: clinical_information Experimental Factor Type: growth_condition
Project description:The HipSci project brings together diverse constituents in genomics, proteomics, cell biology and clinical genetics to create a UK national iPS cell resource and use it to carry out cellular genetic studies. In this sub-study we perform RNAseq on iPS cells generated from skin biopsies from healthy volunteers. This data is part of a pre-publication release. For information on the correct use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/