Genomics

Dataset Information

416

TraDIS to test MICROME single carbon source


ABSTRACT: http://www.sanger.ac.uk/resources/downloads/bacteria/salmonella.html

INSTRUMENT(S): Illumina HiSeq 2000

SUBMITTER: The Wellcome Trust Sanger Institute  

PROVIDER: E-ERAD-5 | ArrayExpress | 2013-06-26

SECONDARY ACCESSION(S): ERP000648

REPOSITORIES: ArrayExpress, ENA

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Publications


Very high-throughput sequencing technologies need to be matched by high-throughput functional studies if we are to make full use of the current explosion in genome sequences. We have generated a very large bacterial mutant pool, consisting of an estimated 1.1 million transposon mutants and we have used genomic DNA from this mutant pool, and Illumina nucleotide sequencing to prime from the transposon and sequence into the adjacent target DNA. With this method, which we have called TraDIS (transpo  ...[more]

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