Mapping translocation breakpoints by next-generation sequencing
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA108267
ORGANISM(S): Homo sapiens
SUBMITTER: 
Reinhard Ullmann
PROVIDER: E-GEOD-10115 | ArrayExpress | 2010-05-22
SECONDARY ACCESSION(S): GSE10115PRJNA108267
REPOSITORIES: GEO, ArrayExpress
Dataset's files
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| E-GEOD-10115.README.txt | Txt | |||
| E-GEOD-10115.biosamples.map | Other | |||
| E-GEOD-10115.biosamples.png | Other | |||
| E-GEOD-10115.biosamples.svg | Svg | |||
| E-GEOD-10115.eSet.r | Other |
Items per page: 5 1 - 5 of 12 |
Publications
Mapping translocation breakpoints by next-generation sequencing.
Chen Wei W Kalscheuer Vera V Tzschach Andreas A Menzel Corinna C Ullmann Reinhard R Schulz Marcel Holger MH Erdogan Fikret F Li Na N Kijas Zofia Z Arkesteijn Ger G Pajares Isidora Lopez IL Goetz-Sothmann Margret M Heinrich Uwe U Rost Imma I Dufke Andreas A Grasshoff Ute U Glaeser Birgitta B Vingron Martin M Ropers H Hilger HH
Genome research 20080307 7
Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterization of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labeled bacterial artificial chromosome clones (BAC-FISH), is rather laborious and time-consuming. In additio ...[more]
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2008-05-11 | E-GEOD-7527 | ArrayExpress
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Project description:Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterization of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labeled bacterial artificial chromosome clones (BAC-FISH), is rather laborious and time-consuming. In addition, the resolution of BAC-FISH is often insufficient to unequivocally identify the disrupted gene. To overcome these limitations, we have performed shotgun sequencing of flow-sorted derivative chromosomes using "next-generation" (Illumina/Solexa) multiplex sequencing-by-synthesis technology. As shown here for three different disease-associated BCRs, the coverage attained by this platform is sufficient to bridge the breakpoints by PCR amplification, and this procedure allows the determination of their exact nucleotide positions within a few weeks. Its implementation will greatly facilitate large-scale breakpoint mapping and gene finding in patients with disease-associated balanced translocations.
2008-01-01 | S-EPMC2493403 | BioStudies
Project description:Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterisation of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labeled bacterial artificial chromosome clones (BAC-FISH), is rather laborious and time consuming. In addition, the resolution of BAC-FISH is often insufficient to unequivocally identify the disrupted gene. To overcome these limitations, we have performed shotgun sequencing of flow-sorted derivative chromosomes using ‘next generation’ (Solexa/Illumina) multiplex sequencing-by-synthesis technology. As shown here for three different disease-associated BCRs, the coverage attained by this platform is sufficient to bridge the breakpoints by PCR amplification, and this procedure allows to determine their exact nucleotide positions within few weeks. Its implementation will greatly facilitate large-scale breakpoint mapping and gene finding in patients with disease-associated balanced translocations. Keywords: Array CGH Overall design: Array CGH was performed in three carriers of balanced translocations to exclude DNA copy number changes.
2008-04-01 | GSE10115 | GEO