Transcriptomics,Multiomics

Dataset Information

2

Mapping translocation breakpoints by next-generation sequencing


ABSTRACT: Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterisation of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labeled bacterial artificial chromosome clones (BAC-FISH), is rather laborious and time consuming. In addition, the resolution of BAC-FISH is often insufficient to unequivocally identify the disrupted gene. To overcome these limitations, we have performed shotgun sequencing of flow-sorted derivative chromosomes using ‘next generation’ (Solexa/Illumina) multiplex sequencing-by-synthesis technology. As shown here for three different disease-associated BCRs, the coverage attained by this platform is sufficient to bridge the breakpoints by PCR amplification, and this procedure allows to determine their exact nucleotide positions within few weeks. Its implementation will greatly facilitate large-scale breakpoint mapping and gene finding in patients with disease-associated balanced translocations. Array CGH was performed in three carriers of balanced translocations to exclude DNA copy number changes.

OTHER RELATED OMICS DATASETS IN: PRJNA108267

ORGANISM(S): Homo sapiens  

SUBMITTER: Reinhard Ullmann  

PROVIDER: E-GEOD-10115 | ArrayExpress | 2010-05-22

SECONDARY ACCESSION(S): GSE10115PRJNA108267

REPOSITORIES: GEO, ArrayExpress

Dataset's files

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Action DRS
E-GEOD-10115.README.txt Txt
E-GEOD-10115.biosamples.map Other
E-GEOD-10115.biosamples.png Other
E-GEOD-10115.biosamples.svg Svg
E-GEOD-10115.eSet.r Other
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Publications


Balanced chromosome rearrangements (BCRs) can cause genetic diseases by disrupting or inactivating specific genes, and the characterization of breakpoints in disease-associated BCRs has been instrumental in the molecular elucidation of a wide variety of genetic disorders. However, mapping chromosome breakpoints using traditional methods, such as in situ hybridization with fluorescent dye-labeled bacterial artificial chromosome clones (BAC-FISH), is rather laborious and time-consuming. In additio  ...[more]

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