Dataset Information


GATA-1-regulated erythroid microRNAs

ABSTRACT: We employed a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. To search for GATA-1-regulated erythroid miRNAs, we used the Gata-1– erythroblast line G1E. These cells proliferate in culture as immature erythroid precursors and undergo terminal maturation when GATA-1 activity is restored. G1E-ER4 is a sub-line stably expressing an estrogen-activated form of GATA-1 (GATA-1 fused to the ligand binding domain of the estrogen receptor). Treatment of G1E-ER4 cells with estradiol induces a GATA-1-regulated program of gene expression with concomitant cellular maturation. We used a microarray to evaluate the expression of 292 different miRNAs in G1E-ER4 cells at 0 versus 24 hours after GATA-1 activation. Affymetrix gene expression profiling has previously been deposited (GEO accession no. GSE628). Keywords: microRNA analysis of a cell-line model of erythroid maturation Two condition experiment, 3 replicates each (independently grown and harvested) of untreated and estradiol-treated (24hrs) G1E-ER4 cells, which express an estrogen-responsive form of the GATA-1 transcription factor. Each sample is compared to a common reference sample, comprised of an equal mixture of all 6 experimental samples.

ORGANISM(S): Mus musculus  

SUBMITTER: Louis C Dore   Mitchell J Weiss 

PROVIDER: E-GEOD-10134 | ArrayExpress | 2008-02-05



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A GATA-1-regulated microRNA locus essential for erythropoiesis.

Dore Louis C LC   Amigo Julio D JD   Dos Santos Camila O CO   Zhang Zhe Z   Gai Xiaowu X   Tobias John W JW   Yu Duonan D   Klein Alyssa M AM   Dorman Christine C   Wu Weisheng W   Hardison Ross C RC   Paw Barry H BH   Weiss Mitchell J MJ  

Proceedings of the National Academy of Sciences of the United States of America 20080226 9

MicroRNAs (miRNAs) control tissue development, but their mechanism of regulation is not well understood. We used a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. Two conserved miRNAs, miR 144 and miR 451, emerged as direct targets of the critical hematopoietic transcription factor GATA-1. In vivo, GATA-1 binds a distal upstream regulatory element to activate RNA polymerase II-mediated transcription of  ...[more]

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