Dataset Information


Lactococcus lactis stringent response

ABSTRACT: The stringent response was defined in Lactococcus lactis through transcript profiling after the addition of a chemical inductor, the norvaline. Gene expression was measured in the exponential growth phase (reference sample) and at 1.6 h after norvaline addition. Four hundred and sixty one differentially expressed genes were identified and constituted the stringent response regulon. Keywords: stress response, time course Stringent response was imposed through norvaline addition during the growth of Lactococcus lactis IL1403 under controlled conditions (30 °C, pH 6.6, nitrogen atmosphere). Cell samples were harvested in exponential phase and 1.6 h after norvaline addition. Total RNA was extracted from these samples and radiolabelled cDNA were prepared and hybridized on nylon arrays. 2053 amplicons specific of Lactococcus lactis IL1403 genes were spotted twice on the array. The 2 time-points were analyzed simultaneously and 3 independent repetitions were performed.

ORGANISM(S): Lactococcus lactis  

SUBMITTER: Muriel Cocaign-Bousquet   Emma Redon  Pascal Loubiere 

PROVIDER: E-GEOD-10254 | ArrayExpress | 2010-06-25



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Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses.

Dressaire Clémentine C   Redon Emma E   Milhem Helene H   Besse Philippe P   Loubière Pascal P   Cocaign-Bousquet Muriel M  

BMC genomics 20080721

BACKGROUND: The development of transcriptomic tools has allowed exhaustive description of stress responses. These responses always superimpose a general response associated to growth rate decrease and a specific one corresponding to the stress. The exclusive growth rate response can be achieved through chemostat cultivation, enabling all parameters to remain constant except the growth rate. RESULTS: We analysed metabolic and transcriptomic responses of Lactococcus lactis in continuous cultures a  ...[more]

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