Dataset Information


Highly integrated epigenome maps in Arabidopsis - whole genome shotgun bisulfite sequencing

ABSTRACT: Part of a set of highly integrated epigenome maps for Arabidopsis thaliana. Keywords: Illumina high-throughput bisulfite sequencing Whole genome shotgun bisulfite sequencing of wildtype Arabidopsis plants (Columbia-0), and met1, drm1 drm2 cmt3, and ros1 dml2 dml3 null mutants using the Illumina Genetic Analyzer.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Ronan C O'Malley   Charles C Berry  Joseph R Ecker  Julian Tonti-Filippini  Brian D Gregory  A H Millar  Ryan Lister 

PROVIDER: E-GEOD-10966 | ArrayExpress | 2008-04-21



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Highly integrated single-base resolution maps of the epigenome in Arabidopsis.

Lister Ryan R   O'Malley Ronan C RC   Tonti-Filippini Julian J   Gregory Brian D BD   Berry Charles C CC   Millar A Harvey AH   Ecker Joseph R JR  

Cell 20080501 3

Deciphering the multiple layers of epigenetic regulation that control transcription is critical to understanding how plants develop and respond to their environment. Using sequencing-by-synthesis technology we directly sequenced the cytosine methylome (methylC-seq), transcriptome (mRNA-seq), and small RNA transcriptome (smRNA-seq) to generate highly integrated epigenome maps for wild-type Arabidopsis thaliana and mutants defective in DNA methyltransferase or demethylase activity. At single-base  ...[more]

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