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Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum

ABSTRACT: Analysis of a Bradyrhizobium japonicum pmtA mutant. PmtA catalyzes the first of three consecutive methylation reactions leading to phosphatidylcholine (PC) formation in B. japonicum. Disruption of the pmtA gene results in a significantly reduced PC content causing a defect in symbiosis with the soybean host. This study provides the first insight into global transcriptomic changes of a bacterial phosphatidylcholine biosynthesis mutant. Cells of the pmtA mutant and the wild type were grown to mid-exponential phase in full medium (PSY) under aerobic culture conditions. Keywords: genetic modification Comparative analyis of the B. japonicum pmtA mutant and the wild type grown under aerobic culture conditions.

ORGANISM(S): Bradyrhizobium japonicum  

SUBMITTER: S Hacker   F Narberhaus  Stefan Zoller  G Pessi  A Lindemann  S Mesa  H.M Fischer  H Hennecke 

PROVIDER: E-GEOD-11187 | ArrayExpress | 2008-04-17



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Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum.

Hacker Stephanie S   Gödeke Julia J   Lindemann Andrea A   Mesa Socorro S   Pessi Gabriella G   Narberhaus Franz F  

Molecular genetics and genomics : MGG 20080430 1

Phosphatidylcholine (PC) is the major phospholipid in eukaryotic membranes. In contrast, it is found in only a limited number of bacteria including members of the Rhizobiales. Here, PC is required for pathogenic and symbiotic plant-microbe interactions, as shown for Agrobacterium tumefaciens and Bradyrhizobium japonicum, respectively. Two different phospholipid N-methyltransferases, PmtA and PmtX1, convert phosphatidylethanolamine (PE) to PC by three consecutive methylation reactions in B. japon  ...[more]

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