Transcriptomics

Dataset Information

2

Brucella melitensis global gene expression


ABSTRACT: In this study we report that B. melitensis at the late logarithmic phase of growth are more invasive for HeLa cells than at mid logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase compared to the stationary growth phase. The vast majority of the up-regulated genes in late-log cultures were those associated with DNA replication, transcription and translation, intermediate metabolism, energy production and conversion, membrane transport and cell envelope, biogenesis and outer membrane, while the down-regulated genes were distributed among several functional categories. This first Brucella global gene expression study provides novel information on growth phase-specific gene regulation important not only for understanding Brucella physiology but also the initial molecular interactions between Brucella and its host. Keywords: Comparison bacterial growth phase normalized to genomic DNA There are two kind of samples consisting of RNA isolated from Brucella melitensis grown logarithmically or at stationary phase. There are four biological replicates of each sample. Every Brucella melitensis open reading frame was printed in triplicate on each microarray, thereby providing three technical replicates for each biological replicates. Each replicate was normalized against labeled Brucella melitensis genomic DNA.

ORGANISM(S): Brucella melitensis  

SUBMITTER: Cristi L Galindo   Harold R Garner  Leslie Garry Adams  Carlos A Rossetti  L G Adams  Thomas A Ficht  Sara D Lawhon 

PROVIDER: E-GEOD-11192 | ArrayExpress | 2010-05-19

SECONDARY ACCESSION(S): GSE11192PRJNA106781

REPOSITORIES: GEO, ArrayExpress

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Publications

Brucella melitensis global gene expression study provides novel information on growth phase-specific gene regulation with potential insights for understanding Brucella:host initial interactions.

Rossetti Carlos A CA   Galindo Cristi L CL   Lawhon Sara D SD   Garner Harold R HR   Adams L Garry LG  

BMC microbiology 20090506


Brucella spp. are the etiological agents of brucellosis, a zoonotic infectious disease that causes abortion in animals and chronic debilitating illness in humans. Natural Brucella infections occur primarily through an incompletely defined mechanism of adhesion to and penetration of mucosal epithelium. In this study, we characterized changes in genome-wide transcript abundance of the most and the least invasive growth phases of B. melitensis cultures to HeLa cells, as a preliminary approach for i  ...[more]

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