Transcriptomics

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Transcription profiling of mouse KCl depolarization-regulated genes in cortical neurons


ABSTRACT: we used DNA microarray analysis to identify genes that are induced by neuronal activity in excitatory neurons at the time when inhibitory synapses are forming and maturing on them. Experiment Overall Design: We cultured cortical neurons for 7 DIV until the process of inhibitory synapse development was underway, and then depolarized the neurons with 50 mM of KCl to activate L-type voltage-sensitive calcium channels (L-VSCCs) for 0, 1 or 6 hours, the cells were lysed, mRNA isolated and hybridized to Affymetrix arrays. Data were collected from 3 independent experiments.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

SUBMITTER: Yingxi Lin   

PROVIDER: E-GEOD-11256 | ArrayExpress | 2008-10-26

SECONDARY ACCESSION(S): GSE11256PRJNA109193

REPOSITORIES: GEO, ArrayExpress

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