Transcriptomics

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Mucosal responses of healthy humans to exponentially growing or stationary Lactobacillus plantarum bacteria


ABSTRACT: Some commensal bacteria stimulate the immune system but do not present specific antigenicity. Such adjuvant effects have been reported for the bacterial species Lactobacillus plantarum. To study in vivo human responses to L. plantarum, a randomised double-blind placebo-controlled cross-over study was performed. Healthy adults were provided preparations of living and heat-killed L. plantarum bacteria, biopsies were taken from the intestinal mucosa and altered transcriptional profiles were analysed. Transcriptional profiles of human epithelia displayed striking differences upon exposure to living L. plantarum bacteria harvested at different growth phases. Modulation of NF-κB-dependent pathways was central among the major altered cellular responses. This unique in vivo study shows which cellular pathways are associated with the induction of immune tolerance in mucosal tissues towards common adjuvanticity possessing lactobacilli. Keywords: mucosal response of healthy adult humans to lactic acid bacteria This study was set up according to a randomised double-blind cross-over placebo-controlled design. It contains transcriptional profiles from biopsies from 8 healthy individuals after oral intake of three different growth stages of Lactobacillus plantarum or placebo control. In total, this study includes data from 8 individuals x 4 treatments=32 arrays.

ORGANISM(S): Homo sapiens  

SUBMITTER: Peter van Baarlen   Fred J Troost  Michiel Kleerebezem  Cindy van der Meer  Guido Hooiveld  Robert-Jan M Brummer  Philip J de Groot 

PROVIDER: E-GEOD-11355 | ArrayExpress | 2009-01-20

SECONDARY ACCESSION(S): GSE11355PRJNA106573

REPOSITORIES: GEO, ArrayExpress

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Publications

Differential NF-kappaB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance.

van Baarlen Peter P   Troost Freddy J FJ   van Hemert Saskia S   van der Meer Cindy C   de Vos Willem M WM   de Groot Philip J PJ   Hooiveld Guido J E J GJ   Brummer Robert-Jan M RJ   Kleerebezem Michiel M  

Proceedings of the National Academy of Sciences of the United States of America 20090203 7


How do we acquire immune tolerance against food microorganisms and commensal bacteria that constitute the intestinal microbiota? We investigated this by stimulating the immune system of adults with commensal Lactobacillus plantarum bacteria. We studied the in vivo human responses to L. plantarum in a randomized double-blind placebo-controlled cross-over study. Healthy adults ingested preparations of living and heat-killed L. plantarum bacteria. Biopsies were taken from the intestinal duodenal mu  ...[more]

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