Transcriptomics

Dataset Information

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Transcription profiling of human HCMV-Infected monocytes study 2


ABSTRACT: Human cytomegalovirus induces a pro-inflammatory monocyte following infection. To begin to address how HCMV induces these rapid changes in infected monocytes, we examined the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed a significant number of pro-inflammatory genes were upregulated within 4 hours post infection. Experiment Overall Design: To begin to globally define the HCMV-induced changes in monocyte function, we performed a transcriptome analysis. Specifically, a cDNA microarray containing 12,626 unique probe sets was utilized to assess the modulation of the monocyte transcriptome at 4 hours post infection. A total of 6 replicates from mock-infected and 6 replicates from HCMV-infected monocytes were analyzed in this study.

REANALYSED by: E-GEOD-11408

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Andrew D. Yurochko  

PROVIDER: E-GEOD-11408 | ArrayExpress | 2008-06-20

SECONDARY ACCESSION(S): GSE11408PRJNA106539

REPOSITORIES: GEO, ArrayExpress

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Publications

Transcriptome analysis reveals human cytomegalovirus reprograms monocyte differentiation toward an M1 macrophage.

Chan Gary G   Bivins-Smith Elizabeth R ER   Smith M Shane MS   Smith Patrick M PM   Yurochko Andrew D AD  

Journal of immunology (Baltimore, Md. : 1950) 20080701 1


Monocytes are primary targets for human CMV (HCMV) infection and are proposed to be responsible for hematogenous dissemination of the virus. Monocytes acquire different functional traits during polarization to the classical proinflammatory M1 macrophage or the alternative antiinflammatory M2 macrophage. We hypothesized that HCMV induced a proinflammatory M1 macrophage following infection to promote viral dissemination because, biologically, a proinflammatory state provides the tools to drive inf  ...[more]

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