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Discovery of the targets of the immune response to chronic myeloid leukemia in 3 patients using protein microarrays

ABSTRACT: Three patients who had relapsed chronic myeloid leukemia after allogeneic bone marrow transplant and received an immunotherapeutic intervention (donor lymphocyte infusion, or DLI) without further treatment were studied. The binding of serum immunoglobulins to proteins after immunotherapy was compared to before in two patients using Invitrogen ProtoArrays from a single lot. For a third patient, comparison was made between two timepoints after immunotherapy against one timepoint before using Invitrogen ProtoArrays from a separate lot. Significant interactions were determined by comparing each after sample separately against the before sample from that patient, using the Concentration-Dependent Analysis described in Marina et al., J Proteome Res, 2008. The goal was to identify proteins with significantly-increased reactivity after donor lymphocyte infusion. Keywords: Immune response discovery One sample from before and one sample from after immunotherapy were tested for 2 patients, and one sample from before and two samples from after immunotherapy were tested for a third patient.

ORGANISM(S): Homo sapiens  

SUBMITTER: Ovidiu Marina  

PROVIDER: E-GEOD-11565 | ArrayExpress | 2008-05-31



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Efficacious immune therapy in chronic myelogenous leukemia (CML) recognizes antigens that are expressed on CML progenitor cells.

Biernacki Melinda A MA   Marina Ovidiu O   Zhang Wandi W   Liu Fenglong F   Bruns Ingmar I   Cai Ann A   Neuberg Donna D   Canning Christine M CM   Alyea Edwin P EP   Soiffer Robert J RJ   Brusic Vladimir V   Ritz Jerome J   Wu Catherine J CJ  

Cancer research 20100126 3

Curative effects of graft-versus-leukemia-based therapies such as donor lymphocyte infusion (DLI) for chronic myelogenous leukemia (CML) may result from immunologic ablation of self-renewing CML progenitor cells. Patients who achieved durable remissions after DLI developed a significant B-cell lymphocytosis after treatment, which did not occur in patients who were unresponsive to DLI. In this study, we identified antigen targets of this B-cell response by probing two immunoproteomic platforms wi  ...[more]

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