Time course RA-treatment of B16 mouse melanoma cells
ABSTRACT: Time course experiment for treatment of B16 mouse melanoma cells with all-trans retinoic acid Keywords: Time couse Time-course experiment with treatment at 4hr, 10hr, 24hr, and 48hr. Six biological replicates per time point; one biological replicate per array. Dye swap.
Project description:Time course experiment for treatment of B16 mouse melanoma cells with all-trans retinoic acid Keywords: Time couse Overall design: Time-course experiment with treatment at 4hr, 10hr, 24hr, and 48hr. Six biological replicates per time point; one biological replicate per array. Dye swap.
Project description:This SuperSeries is composed of the following subset Series: GSE11580: Time course RA-treatment of B16 mouse melanoma cells GSE11584: Melan-a mouse melanocytes vs. B16 mouse melanoma cells Keywords: SuperSeries Refer to individual Series
Project description:Gene expression profile of melan-a mouse melanocytes vs. B16 mouse melanoma cells. Experiment Overall Design: Balanced block design with dye swap. Six biological replicates, one replicate per array.
Project description:Tumor resistance to anti-cancer drugs is a major huddle in chemotherapy. To identify cancer genes that contribute to chemoresistance, B16 mouse melanoma cells were used as a model. We used microarrays to decipher the specific gene regulation in doxorubicine treated B16 mouse melanoma cells. Keywords: Time course Overall design: B16 cells were treated with 1 microgram/ml of doxorubicine and the RNAs were purified at 0, 2, and 4h after the treatment.
Project description:Transcriptional profiling of Candida albicans that had been phagocytosed for 1, 2, or 4 hours by either bone marrow-derived mouse macrophaged (BMDM) or immortalized RAW264.7 cells Keywords: Time course Two-condition experiment, phagocytosed cells vs cells maintained in D-10 media. Time course: 1, 2, and 4 hours. Biological replicates: 4 independent experiments at each cell-time combinations.
Project description:In order to analyse the B16-MDSCs proteome in a large-scale format, we used a multi-dimensional fractionation approach which combines peptide chromatographic fractionation strategies coupled to mass spectrometry.
Project description:In this study, we examined the differential RNA profile of LY500307-treated B16 cells compared with control-treated B16 cells Overall design: We used RNA sequencing to compare the differential RNAs of LY500307-treated B16 cells compared with control-treated B16 cells
Project description:Transcriptional profiling of N-Tera2 differentiated human neuronal cells, comparing control uninfected cells to HCoV-OC43 infected cells at 24, 48 and 72 hour post-infection Keywords: Cell response to viral infection Two-condition experiment, N-Tera2 differentiated human neuronal cell mock infected vs. N-Tera2 differentiated human neuronal cell HCoV-OC43 infected at 24, 48 and 72 hours. Biological replicates: 2 at each time-course point. Technical replicate: 2 dye-swap at each time-point. 2 arrays hybridized with mock(cy3) vs infected(cy5) and 2 array with infected(cy3) vs mock(cy5).
Project description:Analysis of B16 tumor microenvironments at gene expression level. The hypothesis tested in the present study was that Tregs orchastrated the immune reponse triggered in presence of tumors Total RNA obtained from tumor microenvironment tissues (at Day 4 and Day 14 after tumor inoculation) compared to control tissues.
Project description:Gene expression signature of Treg cells in B16 melanoma was measured and compared to B16-infiltrating CD4+ Tconv cells and CD8+ T cells as well as splenic Treg cells, CD4+ Tconv cells and CD8+ T cells. Overall design: We isolated regulatory and conventional CD4+ T cells as well as CD8+ T cells from B16 melanoma and spleen of mice. Cells were isolated, pooled and target cells were purified by FACS. RNA was extracted and gene expression measured.