Transcriptomics

Dataset Information

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Transcription profiling by array of Drosophila Roo lines fed on a diet containing lead acetate


ABSTRACT: 75 Diosophilia Roo lines, recominant inbred lines, were raised to adulthood either on conventional diet or diet supplemented with lead acetate. Whole flies were used for RNA extraction. RNA was run on Dros Genome 2 arrays. Each line also genotyped for 88 markers; Control food consisted of standard cornmeal, agar, sugar, yeast, and 250 microM NaAc (Ashburner 1989). Lead-contaminated food consisted of standard food plus 250 microM PbAc (lead exposure at this concentration has been shown to affect locomotion in adults (Hirsch et al. 2003)). Experiment Overall Design: 75 RI lines each line treated and untreated with lead acetate

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Grier Page  

PROVIDER: E-GEOD-11695 | ArrayExpress | 2009-06-08

SECONDARY ACCESSION(S): GSE11695PRJNA106103

REPOSITORIES: GEO, ArrayExpress

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Publications

Genetical toxicogenomics in Drosophila identifies master-modulatory loci that are regulated by developmental exposure to lead.

Ruden Douglas M DM   Chen Lang L   Possidente Debra D   Possidente Bernard B   Rasouli Parsa P   Wang Luan L   Lu Xiangyi X   Garfinkel Mark D MD   Hirsch Helmut V B HV   Page Grier P GP  

Neurotoxicology 20090906 6


The genetics of gene expression in recombinant inbred lines (RILs) can be mapped as expression quantitative trait loci (eQTLs). So-called "genetical genomics" studies have identified locally acting eQTLs (cis-eQTLs) for genes that show differences in steady-state RNA levels. These studies have also identified distantly acting master-modulatory trans-eQTLs that regulate tens or hundreds of transcripts (hotspots or transbands). We expand on these studies by performing genetical genomics experiment  ...[more]

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