Transcriptomics

Dataset Information

4

Mutant analysis of C. elegans small RNAs, 5p-end-independent


ABSTRACT: These datasets profile the expression of small RNAs with 3p hydroxyls (including endo-siRNAs, miRNAs, and 21U-RNAs) in young adult worms from the wt and prg-1 mutant backgrounds. They were prepared using an approach that captures small RNAs independent of the covalent status of their 5p termini, as described in Ambros et al. (2003) Curr Biol 13:807-18. These datasets were prepared in the Craig C. Mello laboratory. Keywords: endo-siRNA mutant expression profiling CE-prg1-endoSiRNAs-Illumina 1 flowcell each for the wt and prg-1 mutant

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: James C Carrington   Shenghua Duan  Julie M Claycomb  Daniel A Chaves  Jessica J Vasale  David P Bartel  Gary P Schroth  Noah Fahlgren  James Graham Ruby  Craig C Mello  Pedro J Batista  Shujun Luo  Darryl Conte  Kristin D Kasschau  Rosaria Chiang  J G Ruby  Weifung Gu 

PROVIDER: E-GEOD-11736 | ArrayExpress | 2008-06-15

SECONDARY ACCESSION(S): GSE11736PRJNA109171

REPOSITORIES: GEO, ArrayExpress

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Publications


In metazoans, Piwi-related Argonaute proteins have been linked to germline maintenance, and to a class of germline-enriched small RNAs termed piRNAs. Here we show that an abundant class of 21 nucleotide small RNAs (21U-RNAs) are expressed in the C. elegans germline, interact with the C. elegans Piwi family member PRG-1, and depend on PRG-1 activity for their accumulation. The PRG-1 protein is expressed throughout development and localizes to nuage-like structures called P granules. Although 21U-  ...[more]

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