Dataset Information


Transcription profiling of mouse naive T cells isolated from CD4+ CD62L+ CD44- T cells were isolated from Fur flox/flox and CD4 cre Fur flox/flox mice reveals T cell-expressed proprotein convertase furin is essential for maintenance of peripheral tolerance

ABSTRACT: Furin is a proprotein convertase induced in activated T cells, reported to processes the anti-inflammatory cytokine TGFb-1. Herein, we show that conditional deletion of furin in T cells allowed for normal T cell development but impaired the function of regulatory T cells and effector cells, which produced less TGFb-1. Furin-deficient Treg cells, were less protective in a T cell transfer colitis model and failed to induce Foxp3 in normal T cells. Furin-deficient effector cells were inherently overly active and were resistant to suppressive activity of wild-type Tregs. Thus, our results indicate that furin is indispensable in maintaining peripheral tolerance, which is due, at least in part, to its nonredundant, essential function in regulating TGFb-1 production. Targeting furin has emerged as a strategy in malignant and infectious disease. The current work suggests that inhibiting furin might activate immune responses, but may result in a breakdown in peripheral tolerance. Experiment Overall Design: Naive CD4+ CD62L+ CD44- T cells were isolated from Fur flox/flox and CD4 cre Fur flox/flox mice. Replicated samples were achieved for wild type and knockout conditions.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  


PROVIDER: E-GEOD-11884 | ArrayExpress | 2008-10-21



Similar Datasets

2008-06-26 | GSE11884 | GEO
2015-08-05 | E-GEOD-67884 | ArrayExpress
2008-06-27 | E-MEXP-1682 | ArrayExpress
2011-02-19 | E-GEOD-27416 | ArrayExpress
2011-02-19 | GSE27416 | GEO
2012-01-01 | S-EPMC3444655 | BioStudies
2016-01-01 | S-EPMC4860725 | BioStudies
2012-01-01 | S-EPMC3359417 | BioStudies
| GSE84117 | GEO
1000-01-01 | S-EPMC5648857 | BioStudies