Transcriptomics

Dataset Information

61

Small RNAs in rice seed


ABSTRACT: Small RNAs are common and effective modulators of gene expression in eukaryotic organisms. To characterize the small RNAs expressed during rice seed development, massively parallel signature sequencing (MPSS) was performed, resulting in the obtainment of 22-nt sequence signatures. Through integrative analysis,novel miRNAs were identified mostly based on the miRNA* accumulation. Total RNA was isolated separately from rice seeds collected at 3, 6, 9 and 12 days after anthesis (DAA), then mixed into a library and separated on denatured polyacrylamide gel electrophoresis (PAGE). The fraction of 18-26 nt small RNA was recovered by small RNA gel extraction Kit.

ORGANISM(S): Oryza sativa  

SUBMITTER: Liang-Jiao Xue   Jing-Jing Zhang  Hong-Wei Xue 

PROVIDER: E-GEOD-11974 | ArrayExpress | 2009-02-02

SECONDARY ACCESSION(S): GSE11974PRJNA105849

REPOSITORIES: GEO, ArrayExpress

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Publications

Characterization and expression profiles of miRNAs in rice seeds.

Xue Liang-Jiao LJ   Zhang Jing-Jing JJ   Xue Hong-Wei HW  

Nucleic acids research 20081222 3


Small RNAs (sRNAs) are common and effective modulators of gene expression in eukaryotic organisms. To characterize the sRNAs expressed during rice seed development, massively parallel signature sequencing (MPSS) was performed, resulting in the obtainment of 797,399 22-nt sequence signatures, of which 111,161 are distinct ones. Analysis on the distributions of sRNAs on chromosomes showed that most sRNAs originate from interspersed repeats that mainly consist of transposable elements, suggesting t  ...[more]

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