Transcriptomics

Dataset Information

3

Genome-associated RNA Polymerase II includes the dissociable Rpb4/7 subcomplex


ABSTRACT: Yeast RNA polymerase (Pol) II consists of a ten-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immuno-precipitation coupled to high-resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle. Keywords: ChIP-chip Comparison of Rpb3 vs. Rpb4/7 genome-wide occupancy profiles. Data obtained from independent ChIP-chip experiments on two yeast strains: S288C Rpb3TAP vs. Rpb7-TAP strain and W303 Rpb3-TAP vs. wild type strain (IP with the monoclonal antibody for Rpb4/7). Biological replicates W303 strains: 1 x Rpb3-TAP, 1 x wt with Rpb4/7 antibody, independently grown and harvested. Biological replicates S288C strains: 3 x Rpb3-TAP, 2 x Rpb7-TAP, independently grown and harvested. One replicate per array.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Andrew Flatley   Elena Karakasili  Marian Klaocsay  Anna J. Jasiak  Katja Sträßer  Dietmar E Martin  Patrick Cramer  Johannes Söding  Holger Hartmann  Elisabeth Kremmer  Anna J Jasiak 

PROVIDER: E-GEOD-12060 | ArrayExpress | 2008-07-29

SECONDARY ACCESSION(S): GSE12060PRJNA113393

REPOSITORIES: GEO, ArrayExpress

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Publications

Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.

Jasiak Anna J AJ   Hartmann Holger H   Karakasili Elena E   Kalocsay Marian M   Flatley Andrew A   Kremmer Elisabeth E   Strässer Katja K   Martin Dietmar E DE   Söding Johannes J   Cramer Patrick P  

The Journal of biological chemistry 20080730 39


Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in  ...[more]

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