Transcriptomics

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4

The growth factor environment defines distinct pluripotent ground states in novel blastocyst derived stem cells


ABSTRACT: Pluripotent stem cell lines can be derived from blastocyst embryos, which yield embryonic stem cell lines (ES cells), as well as the post-implantation epiblast, which gives rise to epiblast stem cell lines (EpiSCs). Remarkably, ES cells and EpiSCs display profound differences in the combination of growth factors that maintain their pluripotent state. Molecular and functional differences between these two stem cell types demonstrate that the tissue of origin and/or the growth factor milieu may be important determinants of the stem cell identity. We explored how developmental stage of the tissue of origin and culture growth factor conditions affect the stem cell pluripotent state. Our findings reveal that novel stem cell lines can be generated from blastocyst embryos with unique functional and molecular properties. We demonstrate that the culture growth factor environment and cell-cell interaction play a critical role in defining several unique and stable stem cell ground states. Keywords: Clonal analysis of FAB-SC gene expression Cell type characterization - 3 separate single cell clones

ORGANISM(S): Mus musculus  

SUBMITTER: Joshua G Chenoweth   Niels Geijsen  Akiko Yabuuchi  Maureen Eijpe  Yu-Fen Chou  Hsu-Hsin Chen  R D McKay  Jun Lu 

PROVIDER: E-GEOD-12519 | ArrayExpress | 2010-05-17

SECONDARY ACCESSION(S): GSE12519PRJNA112917

REPOSITORIES: GEO, ArrayExpress

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The growth factor environment defines distinct pluripotent ground states in novel blastocyst-derived stem cells.

Chou Yu-Fen YF   Chen Hsu-Hsin HH   Eijpe Maureen M   Yabuuchi Akiko A   Chenoweth Joshua G JG   Tesar Paul P   Lu Jun J   McKay Ronald D G RD   Geijsen Niels N  

Cell 20081001 3


Pluripotent stem cell lines can be derived from blastocyst embryos, which yield embryonic stem cell lines (ES cells), as well as the postimplantation epiblast, which gives rise to epiblast stem cell lines (EpiSCs). Remarkably, ES cells and EpiSCs display profound differences in the combination of growth factors that maintain their pluripotent state. Molecular and functional differences between these two stem cell types demonstrate that the tissue of origin and/or the growth factor milieu may be  ...[more]

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