Transcriptomics

Dataset Information

5

Hog1, slt2, rlm1, and msn2/4 mutants exposed to zymolyase


ABSTRACT: This SuperSeries is composed of the following subset Series: GSE9930: Wild type 3 hour exposure to zymolyase GSE9931: hog1 mutant 3 hour exposure to zymolyase GSE9932: slt2 mutant 3 hour exposure to zymolyase GSE9933: rlm1 mutant 3 hour exposure to zymolyase GSE9934: msn2/4 mutant 3 hour exposure to zymolyase S. cerevisiae wild type (BY4741) and mutants were grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T. Cells were collected at 3 hours of growth, frozen at -80 °C and processed for RNA extraction. The total RNA from one culture was analyzed, and, in addition, for this sample two different hybridizations were performed, including fluorochrome swapping in order to minimize transcriptional changes due to technical variability. This therefore corresponded to two DNA microarrays analyzed.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Javier Arroyo   Nombela Cesar  Arroyo Javier  Rodriguez-Peña M Jose  Clara Bermejo  Raul Garcia 

PROVIDER: E-GEOD-12684 | ArrayExpress | 2010-06-19

SECONDARY ACCESSION(S): GSE12684PRJNA112685

REPOSITORIES: GEO, ArrayExpress

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Publications

The high osmotic response and cell wall integrity pathways cooperate to regulate transcriptional responses to zymolyase-induced cell wall stress in Saccharomyces cerevisiae.

García Raúl R   Rodríguez-Peña Jose M JM   Bermejo Clara C   Nombela César C   Arroyo Javier J  

The Journal of biological chemistry 20090220 16


The adaptation of Saccharomyces cerevisiae to situations in which cell wall integrity is seriously compromised mainly involves the cell wall integrity (CWI) pathway. However, in a recent work ( Bermejo, C., Rodriguez, E., García, R., Rodríguez-Peña, J. M., Rodríguez de la Concepción, M. L., Rivas, C., Arias, P., Nombela, C., Posas, F., and Arroyo, J. (2008) Mol. Biol. Cell 19, 1113-1124 ) we have demonstrated the co-participation of the high osmotic response (HOG) pathway to ensure yeast surviva  ...[more]

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