Transcriptomics

Dataset Information

16

Expression profiling of Muscle tissue from C57BL/6J X C3H/HeJ)F2 and (C3H/HeJ X C57BL/6J)F2


ABSTRACT: The purpose of this experiment was to determine the expression traits in animals from F2 intercross of inbred strains C57BL/6J, C3H/HeJ. (N=309, 164 males and 145 females). Muscle from 285 F2 female and male mice were generated by intercrossing F1 mice. Mice were fed chow diet containing 4% fat (Ralston-Purina Co., St. Louis, MO) until 8 weeks of age and then were placed on a high-fat "Western" diet containing 42% fat and 0.15% cholesterol (Teklad 88137, Harlan Teklad, Madison WI) for 12 weeks. At 20 weeks mice were sacrificed, after a 12-hour fast Muscle tissues were dissected and flash frozen in LN2 and stored at -80°C. Keywords=Genetics of Gene Expression Keywords Keywords=C57B1/J6 Keywords=C3H/HeJ

ORGANISM(S): Mus musculus  

SUBMITTER: Leslie Ingram-drake  

PROVIDER: E-GEOD-12795 | ArrayExpress | 2009-08-09

SECONDARY ACCESSION(S): GSE12795PRJNA110985

REPOSITORIES: GEO, ArrayExpress

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Publications

Expression quantitative trait loci: replication, tissue- and sex-specificity in mice.

van Nas Atila A   Ingram-Drake Leslie L   Sinsheimer Janet S JS   Wang Susanna S SS   Schadt Eric E EE   Drake Thomas T   Lusis Aldons J AJ  

Genetics 20100503 3


By treating the transcript abundance as a quantitative trait, gene expression can be mapped to local or distant genomic regions relative to the gene encoding the transcript. Local expression quantitative trait loci (eQTL) generally act in cis (that is, control the expression of only the contiguous structural gene), whereas distal eQTL act in trans. Distal eQTL are more difficult to identify with certainty due to the fact that significant thresholds are very high since all regions of the genome m  ...[more]

Publication: 1/2

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