Dataset Information


Transcription profiling of Zebrafish 24hpf mutant sk8

ABSTRACT: The production of functional mRNA involves multiple steps including transcription initiation, elongation, and termination. spt5 encodes a conserved essential transcription elongation factor that controls RNAPII processivity in vitro and co-localizes with RNAPII in vivo. We used microarrays to detail the global expression of gene expression at 24 hours post fertilization in wild-type and Spt5 mutant (fog-sk8) to identify Spt5 transcriptionally dependent genes and affected pathways. Here we report that a mutation in zebrafish spt5, sk8, deregulates <5% of ~10,000 genes examined. These findings reveal a small but diverse set of developmentally regulated genes, whose expression is critically dependent on Spt5-regulated RNAPII stalling in vertebrate development. Experiment Overall Design: RNA from zebrafish embryos were harvested at 24 hours post fertilization for hybridization to Affymetrix gene arrays.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Danio rerio  

SUBMITTER: Nathan George Salomonis  

PROVIDER: E-GEOD-12826 | ArrayExpress | 2008-10-25



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