Dataset Information


Transcription profiling by array of Arabidopsis mutant for ataf1 after infection with Blumeria graminis f.sp. hordei

ABSTRACT: Arabidopsis is a non-host to the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh), effectively hindering Bgh ingress to the epidermal cells by deposition of callosic papillae formations, i.e. penetration resistance. To better understand the transcriptional changes in Arabidopsis towards Bgh, we compared un-inoculated and inoculated wild-type Arabidopsis samples, with those an ATAF1 mutant allele, compromised in penetration resistance. The experiment included sampling of 8 rosettes for each replicate sample from 6-weeks old plants, 12 hrs after inoculation. A total of 12 biolocigal replicates were sampled with three replicates from each of the four blocks (Wild-type, ataf1-1, Bgh, control). ****[PLEXdb( has submitted this series at GEO on behalf of the original contributor, Michael Krogh Jensen. The equivalent experiment is AT31 at PLEXdb.] Experiment Overall Design: genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: Wild-type Arabidopsis, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Un-inoculated(3-replications); genotype: ataf1-1 mutant, ecotype Col-0 - treated or untreated: Bgh inoculated(3-replications)


INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: PLEXdb Curator Sudhansu Dash  

PROVIDER: E-GEOD-12856 | ArrayExpress | 2008-10-25



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Transcriptional regulation by an NAC (NAM-ATAF1,2-CUC2) transcription factor attenuates ABA signalling for efficient basal defence towards Blumeria graminis f. sp. hordei in Arabidopsis.

Jensen Michael K MK   Hagedorn Peter H PH   de Torres-Zabala Marta M   Grant Murray R MR   Rung Jesper H JH   Collinge David B DB   Lyngkjaer Michael F MF  

The Plant journal : for cell and molecular biology 20081004 6

ATAF1 is a member of a largely uncharacterized plant-specific gene family encoding NAC transcription factors, and is induced in response to various abiotic and biotic stimuli in Arabidopsis thaliana. Previously, we showed that a mutant allele of ATAF1 compromises penetration resistance in Arabidopsis with respect to the non-host biotrophic pathogen Blumeria graminis f. sp. hordei (Bgh). In this study, we have used genome-wide transcript profiling to characterize signalling perturbations in ataf1  ...[more]

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