Transcriptomics

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PPARg dependent expression changes during differentiation of 3T3L1 cell into adipocytes


ABSTRACT: We profiled PPARg dependent gene expression changes during differntiation of 3T3L1 cell using PPARg siRNA 3T3-L1 (Pre-adipocyte) cell line was induced to differentiate using standard adipocyte differentiation media (IBMX, Dex and Insulin) 48hrs post-confluency. RNA was harvested at day -2 (confluent fibroblasts), 48hrs post-induction with IBMX, DEX and Insulin (day=0) and for each subsequent day after rosiglitazone treatment. Illumina beadchip microarrays were used to determine expression profiles of genes differentially regulated in cells transfected with either siRNA targeting PPARgamma or a non-targeting control siRNA. 3T3L1 cell were induced to differentiate into adipocytes using IBMX, DEX and Insulin. RNA from cell treated with PPARg-specific siRNA and non-specific siRNA was isolated at different timepoints. Illumina MouseRef-8 v1.1 Bead chips were used for expression profiling

ORGANISM(S): Mus musculus  

SUBMITTER: Vinsensius B Vega   Sebastian Pott  Yijun Ruan  Chia L Wei  Kuo P Chiu  Edison T Liu  Mohamed S Hamza  Gopalan S Kandhadayar  Jane S Thomsen 

PROVIDER: E-GEOD-12929 | ArrayExpress | 2010-04-08

SECONDARY ACCESSION(S): GSE12929PRJNA110849

REPOSITORIES: GEO, ArrayExpress

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Publications


BACKGROUND: The pathophysiology of obesity and type 2 diabetes mellitus is associated with abnormalities in endocrine signaling in adipose tissue and one of the key signaling affectors operative in these disorders is the nuclear hormone transcription factor peroxisome proliferator-activated receptor-gamma (PPARgamma). PPARgamma has pleiotropic functions affecting a wide range of fundamental biological processes including the regulation of genes that modulate insulin sensitivity, adipocyte differ  ...[more]

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