Transcriptomics

Dataset Information

3

Halobacterium salinarum NRC-1 growth curve


ABSTRACT: Halobacterium salinarum NRC-1 was grown in CM media, at 37oC in a waterbath with agitation of 125 rpm under constant light. Analysis of transcriptional changes during growth, in addition to mapping of transcriptome structure under the same conditions, provided interesting insights about regulatory logic within prokaryotic coding regions. Samples were collected at different cell densities, from OD ~0.2 to OD ~5.0. 2 biological replicates were conducted. For each sample, a dye-swap experiment was performed.

ORGANISM(S): Halobacterium salinarum NRC-1  

SUBMITTER: Tie Koide  Min Pan  David J Reiss  Nitin S Baliga  Marc T Facciotti 

PROVIDER: E-GEOD-12977 | ArrayExpress | 2010-05-17

SECONDARY ACCESSION(S): GSE12977PRJNA114245

REPOSITORIES: GEO, ArrayExpress

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Publications


Despite the knowledge of complex prokaryotic-transcription mechanisms, generalized rules, such as the simplified organization of genes into operons with well-defined promoters and terminators, have had a significant role in systems analysis of regulatory logic in both bacteria and archaea. Here, we have investigated the prevalence of alternate regulatory mechanisms through genome-wide characterization of transcript structures of approximately 64% of all genes, including putative non-coding RNAs  ...[more]

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