Transcriptomics

Dataset Information

67

MiRNA analysis of HBMVECs exposed or unexposed to U87 glioma cells


ABSTRACT: A key step in angiogenesis is the upregulation of growth factor receptors on endothelial cells. Here we demonstrate that a small regulatory microRNA, miR-296 has a major role in this process. Glioma cells and angiogenic growth factors elevate the level of miR-296 in primary human brain microvascular endothelial cells in culture. The miR-296 level is also elevated in primary tumor endothelial cells isolated from human brain tumors compared to normal brain endothelial cells. Growth factor-induced miR-296 contributes significantly to angiogenesis by directly targeting the hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) mRNA, leading to decreased levels of HGS and thereby reducing HGS-mediated degradation of the growth factor receptors VEGFR2 and PDGFR-β. Furthermore, inhibition of miR-296 with antagomirs reduces angiogenesis in tumor xenografts in vivo. Keywords: comparitive miRNA analysis 3 biological replicates (HBMVECs) are compared to 3 biological replicates (HBMVECs exposed to U87 glioma cells)

ORGANISM(S): Homo sapiens  

SUBMITTER: Thomas Wurdinger   Anna K Krichevsky 

PROVIDER: E-GEOD-13091 | ArrayExpress | 2010-06-25

SECONDARY ACCESSION(S): GSE13091PRJNA110917

REPOSITORIES: GEO, ArrayExpress

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Publications

miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cells.

Würdinger Thomas T   Tannous Bakhos A BA   Saydam Okay O   Skog Johan J   Grau Stephan S   Soutschek Jürgen J   Weissleder Ralph R   Breakefield Xandra O XO   Krichevsky Anna M AM  

Cancer cell 20081101 5


A key step in angiogenesis is the upregulation of growth factor receptors on endothelial cells. Here, we demonstrate that a small regulatory microRNA, miR-296, has a major role in this process. Glioma cells and angiogenic growth factors elevate the level of miR-296 in primary human brain microvascular endothelial cells in culture. The miR-296 level is also elevated in primary tumor endothelial cells isolated from human brain tumors compared to normal brain endothelial cells. Growth factor-induce  ...[more]

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