Transcriptomics

Dataset Information

299

Mouse Sirt6-/- TNF-alpha timecourse


ABSTRACT: We completed genome-wide microarray analysis in Sirt6 knockout (ko) mouse embryonic fibroblasts (MEFs) and control wildtype (wt) MEFs. Prior to RNA extraction, MEFs were treated with TNF-alpha (10 ng/ml) for the indicated times. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Ambion Amino Allyl MessageAmp II Amplification kit. Amplified mouse Universal Reference RNA (Stratagene) was used as reference RNA for analysis. Array hybridization of mouse MEEBO arrays is as described (Hendrickson et al., PLoS ONE, 2008). Groups of assays that are related as part of a time series. Compound Based Treatment: 10 ng/ml TNF-alpha Keywords: time_series_design Computed

ORGANISM(S): Mus musculus  

SUBMITTER: Tiara Kawahara   Tatiparthi Reddy  Adam S Adler 

PROVIDER: E-GEOD-13207 | ArrayExpress | 2010-06-24

SECONDARY ACCESSION(S): GSE13207PRJNA114029

REPOSITORIES: GEO, ArrayExpress

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Publications

SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span.

Kawahara Tiara L A TL   Michishita Eriko E   Adler Adam S AS   Damian Mara M   Berber Elisabeth E   Lin Meihong M   McCord Ron A RA   Ongaigui Kristine C L KC   Boxer Lisa D LD   Chang Howard Y HY   Chua Katrin F KF  

Cell 20090101 1


Members of the sirtuin (SIRT) family of NAD-dependent deacetylases promote longevity in multiple organisms. Deficiency of mammalian SIRT6 leads to shortened life span and an aging-like phenotype in mice, but the underlying molecular mechanisms are unclear. Here we show that SIRT6 functions at chromatin to attenuate NF-kappaB signaling. SIRT6 interacts with the NF-kappaB RELA subunit and deacetylates histone H3 lysine 9 (H3K9) at NF-kappaB target gene promoters. In SIRT6-deficient cells, hyperace  ...[more]

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