Dataset Information


Mouse Sirt6-/- tissues

ABSTRACT: We harvested liver, spleen, thymus, and skin tissues from 21 or 29 day old Sirt6-/- animals and then completed genome-wide microarray analysis. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Amino Allyl MessageAmp II Amplification kit. Amplified age-matched wild-type tissues were used as reference RNA for analysis. Array hybridization of mouse MEEBO arrays is as described (Hendrickson et al., PLoS ONE, 2008). Set of arrays that are part of repeated experiments Tissue type: Age & tissue harvested Keywords: Biological Replicate Biological Replicate Computed

ORGANISM(S): Mus musculus  

SUBMITTER: Tiara Kawahara   Tatiparthi Reddy  Adam S Adler 

PROVIDER: E-GEOD-13208 | ArrayExpress | 2010-06-24



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SIRT6 links histone H3 lysine 9 deacetylation to NF-kappaB-dependent gene expression and organismal life span.

Kawahara Tiara L A TL   Michishita Eriko E   Adler Adam S AS   Damian Mara M   Berber Elisabeth E   Lin Meihong M   McCord Ron A RA   Ongaigui Kristine C L KC   Boxer Lisa D LD   Chang Howard Y HY   Chua Katrin F KF  

Cell 20090101 1

Members of the sirtuin (SIRT) family of NAD-dependent deacetylases promote longevity in multiple organisms. Deficiency of mammalian SIRT6 leads to shortened life span and an aging-like phenotype in mice, but the underlying molecular mechanisms are unclear. Here we show that SIRT6 functions at chromatin to attenuate NF-kappaB signaling. SIRT6 interacts with the NF-kappaB RELA subunit and deacetylates histone H3 lysine 9 (H3K9) at NF-kappaB target gene promoters. In SIRT6-deficient cells, hyperace  ...[more]

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