Transcriptomics

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Intracellular proliferation and virulence gene expression of Salmonella are influenced by pre-invasion growth conditions


ABSTRACT: Salmonella invasion of non-phagocytic cells is a dynamic process that requires the translocation of effector proteins into host cells by a type III secretion system (T3SS1) encoded on Salmonella pathogenicity island 1 (SPI1). The signals involved in T3SS1 induction in vivo are not known, but SPI1-induced invasive Salmonella can be prepared by growth in synthetic media. Here we compared two different methods of preparing SPI1-induced bacteria by using a combination of transcriptome analysis, a single-cell reporter assay and intracellular gene expression analysis to reveal clear differences between these two bacterial populations that are reflected in their ability to interact with cultured epithelial cells. Invasion efficiency and intracellular replication were greater for bacteria grown with aeration to late-log phase compared to those grown without aeration to stationary phase. No significant differences in SPI1 regulon expression were revealed by transcriptome analysis. By contrast, surface appendage and stress response genes were considerably changed. Single cell analyses of gene expression revealed variation in the frequency of SPI1-induced bacteria and a correlation between SPI1-induction and the presence of flagella. The expression of virulence genes following invasion of cultured epithelial cells was also found to be predetermined by pre-invasion growth conditions. Key words: epithelial, flagella, invasion, motility, Salmonella-containing vacuole, treatment of shaking versus treatment of static growth conditions for St-SL1344

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344  

SUBMITTER: Aaron B Carmody   Dan Sturdevant  Steven F Porcella  Leigh A Knodler  Elizabeth R Fischer  Olivia Steele-Mortimer  Daniel E Sturdevant  J A Ibarra  Kimmo Virtaneva 

PROVIDER: E-GEOD-13343 | ArrayExpress | 2010-04-19

SECONDARY ACCESSION(S): GSE13343PRJNA109865

REPOSITORIES: GEO, ArrayExpress

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Publications

Induction of Salmonella pathogenicity island 1 under different growth conditions can affect Salmonella-host cell interactions in vitro.

Ibarra J Antonio JA   Knodler Leigh A LA   Sturdevant Daniel E DE   Virtaneva Kimmo K   Carmody Aaron B AB   Fischer Elizabeth R ER   Porcella Stephen F SF   Steele-Mortimer Olivia O  

Microbiology (Reading, England) 20091224 Pt 4


Salmonella invade non-phagocytic cells by inducing massive actin rearrangements, resulting in membrane ruffle formation and phagocytosis of the bacteria. This process is mediated by a cohort of effector proteins translocated into the host cell by type III secretion system 1, which is encoded by genes in the Salmonella pathogenicity island (SPI) 1 regulon. This network is precisely regulated and must be induced outside of host cells. In vitro invasive Salmonella are prepared by growth in syntheti  ...[more]

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